anti-Mouse (Murine) HYAL1 antibody for Immunohistochemistry

Recommended HYAL1 Antibody

Antigen
Hyaluronidase-1 (HYAL1) Antibodies
  • HYAL-1
  • LUCA1
  • NAT6
  • Hya1
  • Hyal-1
  • hyaluronoglucosaminidase 1
  • HYAL1
  • Hyal1
Epitope
N-Term
19
15
10
8
6
6
5
5
4
3
3
2
2
2
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
83
39
30
6
5
4
3
2
2
2
1
1
1
Host
Rabbit
90
18
9
Clonality
Polyclonal
Conjugate
This HYAL1 antibody is un-conjugated
9
8
5
4
4
4
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
75
49
40
14
13
8
7
7
3
1
1
1
Options
Successfully validated

Available images

Catalog No. ABIN2784343
$ 319.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
100.65095 ABIN2784342 IHC WB Rabbit N-Term Polyclonal 1
16.093815 ABIN6570575 IHC WB Rabbit IgG Polyclonal 0
16 ABIN3047575 IHC WB Rabbit IgG Polyclonal 0
10.093815 ABIN6744436 IHC IHC (p) WB Rabbit IgG AA 36-85 Polyclonal 0
10 ABIN1859237 ICC IHC WB Rabbit IgG AA 52-462 Polyclonal 0
10 ABIN2933958 IF/ICC IHC IP WB Rabbit AA 52-462 Polyclonal 0
4 ABIN6718271 IHC WB Rabbit Polyclonal 0
1 ABIN5868135 ELISA IHC WB Rabbit Polyclonal 0

General

Antigen Hyaluronidase-1 (HYAL1) Antibodies
Epitope N-Term
(19), (15), (10), (8), (6), (6), (5), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(83), (39), (30), (6), (5), (4), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(90), (18), (9)
Clonality
Polyclonal
Conjugate This HYAL1 antibody is un-conjugated
(9), (8), (5), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(75), (49), (40), (14), (13), (8), (7), (7), (3), (1), (1), (1)
Pubmed 1 reference available
Supplier

Product Details anti-HYAL1 Antibody

Target Details HYAL1 Application Details Handling References for anti-HYAL1 antibody (ABIN2784343) Images
Blocking Peptide Blocking peptide for this product available: ABIN978363
Sequence TIFYSSQLGT YPYYTPTGEP VFGGLPQNAS LIAHLARTFQ DILAAIPAPD
Predicted Reactivity Cow: 86%, Dog: 85%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 92%, Rabbit: 93%, Rat: 92%
Characteristics This is a rabbit polyclonal antibody against HYAL1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human HYAL1
Plasmids, Primers & others

Target Details HYAL1

Product Details anti-HYAL1 Antibody Application Details Handling References for anti-HYAL1 antibody (ABIN2784343) Images back to top
Antigen
Alternative Name HYAL1 (HYAL1 Antibody Abstract)
Background HYAL1 is a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. This gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: HYAL-1, LUCA1, MGC45987, NAT6
Protein Interaction Partner: UBC, COL2A1,
Protein Size: 435
Molecular Weight 45 kDa
Gene ID 3373
NCBI Accession NM_153282, NP_695014
UniProt Q12794
Pathways Glycosaminoglycan Metabolic Process

Application Details

Product Details anti-HYAL1 Antibody Target Details HYAL1 Handling References for anti-HYAL1 antibody (ABIN2784343) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 435 AA

Restrictions For Research Use only

Handling

Product Details anti-HYAL1 Antibody Target Details HYAL1 Application Details References for anti-HYAL1 antibody (ABIN2784343) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HYAL1 antibody (ABIN2784343)

Product Details anti-HYAL1 Antibody Target Details HYAL1 Application Details Handling Images back to top
Background publications

Poola, Abraham, Marshalleck, Yue, Lokeshwar, Bonney, Dewitty: "Molecular risk assessment for breast cancer development in patients with ductal hyperplasias." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 4, pp. 1274-80, 2008

Images

Product Details anti-HYAL1 Antibody Target Details HYAL1 Application Details Handling References for anti-HYAL1 antibody (ABIN2784343) back to top
Supplier Images
Western Blotting (WB) image for anti-Hyaluronidase-1 (HYAL1) (N-Term) antibody (ABIN2784343) WB Suggested Anti-HYAL1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Pos...
Immunohistochemistry (IHC) image for anti-Hyaluronidase-1 (HYAL1) (N-Term) antibody (ABIN2784343) Rabbit Anti-HYAL1 Antibody Formalin Fixed Paraffin Embedded Tissue: Human heart Tis...
'Independent Validation' Badge
Antigen HYAL1 antibody - N-terminal region
Lot Number QC23783
Method validated Immunohistochemistry
Positive Control Testes of Hyal1 +/+ mouse
Negative Control Testes of Hyal1 -/- mouse
Notes ABIN2784343 does detect mouse HYAL1 by immunohistochemistry in paraffin sections. The immunohistochemistry is based on increased signal in wild type mouse testes compared to Hyal1-/- mouse testes.
Primary Antibody ABIN2784343
Secondary Antibody Goat-anti-rabbit AF568
Protocol
  • Deparafinize and rehydrate:
    • xylene (I & II) – 2x 5min
    • 100% Ethanol (I & II) – 2x 5min
    • 95% Ethanol (I & II) – 2x 3min
    • 70% Ethanol (I) – 3min
    • 50% Ethanol (I) – 3min
    • ddH2O – 3min
  • Antigen retrieval:
    • Immerse slides in citrate buffer (10mM Sodium citrate at pH6.0) preheated to 95°C.
    • Incubate at 95°C for 20min.
    • Cool down at RT for 20min.
    • Wash twice with PBS for 5min.
  • Immunostaining:
    • Block in blocking solution (prepare 3% BSA in TBST) at RT for 60min.
    • Incubate sections with primary Hyaluronidase-1 (HYAL1) (N-Term) antibody ABIN2784343 diluted 1:200 in blocking solution.
    • Incubate at 4°C overnight.
    • Wash slides three times with TBST 5min.
    • Incubate sections with goat-anti-rabbit AF568 secondary antibody conjugate diluted 1:500 in blocking solution.
    • Incubate at RT for 1h.
    • Wash slides in TBST - 1x 5min at RT.
    • Wash three times with H2O for 5min.
    • Incubated in Hoechst solution diluted 1:100.000 for 8-9min.
    • Wash twice with H2O for 5min.
    • Mounted with ProLong Gold (ThermoFisher, P36931) solution.
Validation Images
Immunohistochemistry validation image for anti-Hyaluronidase-1 (HYAL1) (N-Term) antibody (ABIN2784343) Testes of Hyal1 -/- mouse (A) or Hyal1 +/+ mouse (B) stained with ABIN2784342 as desc...