anti-Human Hepcidin antibody for ELISA

Recommended Hepcidin Antibody (supplied by: Log in to see )

Antigen
Hepcidin Antimicrobial Peptide (HAMP) Antibodies
  • HEPC
  • HFE2B
  • LEAP1
  • PLTR
  • Hepcidin
  • Hamp1
  • Hepc
  • Hepc1
  • HAMP
  • hepcidin
  • hepcidin antimicrobial peptide
  • hepcidin-like
  • Ham percentage
  • HAMP
  • Hamp
  • hamp
  • LOC100135935
Epitope
AA 25-85
23
10
8
8
7
7
6
5
5
5
5
3
2
2
2
1
1
1
Reactivity
Human
84
19
8
6
5
2
1
Host
Mouse
82
19
14
Clonality
Monoclonal
Conjugate
This Hepcidin antibody is un-conjugated
13
9
3
3
3
3
Application
ELISA, Western Blotting (WB)
94
57
45
19
15
8
5
3
2
1
1
1
1
1
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Catalog No. ABIN394071
$ 461.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.3842535 ABIN566175 ELISA WB Mouse IgG1 kappa AA 25-84, full length Log in to see 1F9 0
1 ABIN1101963 ELISA IHC IHC (p) Goat AA 22-36 Log in to see Polyclonal 0
1 ABIN570711 ELISA WB Goat N-Term Log in to see Polyclonal 0
1 ABIN2464205 ELISA WB Goat N-Term Log in to see Polyclonal 0
1 ABIN574459 ELISA IHC IHC (p) WB Mouse IgG1, kappa AA 25-85 Log in to see 1F9 0
1 ABIN450057 ELISA Goat N-Term Log in to see Polyclonal 1
1 ABIN2605295 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN2605297 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN1735332 IHC (p) ELISA Goat N-Term Log in to see Polyclonal 0
1 ABIN2605296 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2278872 ELISA Alkaline Phosphatase (AP) Goat N-Term Log in to see Polyclonal 0
1 ABIN2278877 ELISA PE Goat N-Term Log in to see Polyclonal 0
1 ABIN2278873 ELISA APC Goat N-Term Log in to see Polyclonal 0
1 ABIN2278874 ELISA Biotin Goat N-Term Log in to see Polyclonal 0
1 ABIN2278875 ELISA FITC Goat N-Term Log in to see Polyclonal 0
1 ABIN2471092 IHC ELISA WB Mouse IgG1 kappa AA 25-85 Log in to see 1F9 0
1 ABIN566173 ELISA WB Mouse AA 25-84, full length Log in to see Polyclonal 0
1 ABIN2278871 ELISA Goat N-Term Log in to see Polyclonal 0
1 ABIN1900976 ELISA WB APC Rabbit IgG AA 17-46 Log in to see Polyclonal 0
1 ABIN1900979 ELISA WB PE Rabbit IgG AA 17-46 Log in to see Polyclonal 0

General

Antigen Hepcidin Antimicrobial Peptide (HAMP) Antibodies
Epitope AA 25-85
(23), (10), (8), (8), (7), (7), (6), (5), (5), (5), (5), (3), (2), (2), (2), (1), (1), (1)
Reactivity Human
(84), (19), (8), (6), (5), (2), (1)
Host Mouse
(82), (19), (14)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Hepcidin antibody is un-conjugated
(13), (9), (3), (3), (3), (3)
Application ELISA, Western Blotting (WB)
(94), (57), (45), (19), (15), (8), (5), (3), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Hepcidin Antibody

Target Details Hepcidin Application Details Handling References for anti-Hepcidin antibody (ABIN394071) Images
Specificity HAMP (AAH20612, 25 a.a. ~ 85 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: HAMP (AAH20612, 25 a.a. ~ 84 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone 1F9
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details Hepcidin

Product Details anti-Hepcidin Antibody Application Details Handling References for anti-Hepcidin antibody (ABIN394071) Images back to top
Antigen
Alternative Name HAMP (HAMP Antibody Abstract)
Background

Other Names: Hepcidin, Liver-expressed antimicrobial peptide 1, LEAP-1, Putative liver tumor regressor, PLTR, Hepcidin-25, Hepc25, Hepcidin-20, Hepc20, HAMP, HEPC, LEAP1

Gene Description: hepcidin antimicrobial peptide

Gene Summary: The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure. [provided by RefSeq

Molecular Weight 9408 DA
Gene ID 57817
NCBI Accession NP_066998
UniProt P81172
Pathways Hormone Activity, Transition Metal Ion Homeostasis

Application Details

Product Details anti-Hepcidin Antibody Target Details Hepcidin Handling References for anti-Hepcidin antibody (ABIN394071) Images back to top
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml
Comment

Background: The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure.

Restrictions For Research Use only

Handling

Product Details anti-Hepcidin Antibody Target Details Hepcidin Application Details References for anti-Hepcidin antibody (ABIN394071) Images back to top
Concentration 0.5 mg/mL
Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-Hepcidin antibody (ABIN394071)

Product Details anti-Hepcidin Antibody Target Details Hepcidin Application Details Handling Images back to top
Background publications

Ucisik-Akkaya, Davis, Do, Morrison, Stemmer, Amadio, Dorak: "Examination of genetic polymorphisms in newborns for signatures of sex-specific prenatal selection." in: Molecular human reproduction, Vol. 16, Issue 10, pp. 770-7, 2010

Lee, Song, Yun, Yoon, Cho, Kim, Lee: "Serum prohepcidin levels in Helicobacter pylori infected patients with iron deficiency anemia." in: The Korean journal of internal medicine, Vol. 25, Issue 2, pp. 195-200, 2010

Kim, Kim, Yoon, Kim, Lee: "Serum pro-hepcidin could reflect disease activity in patients with rheumatoid arthritis." in: Journal of Korean medical science, Vol. 25, Issue 3, pp. 348-52, 2010

De Domenico, Lo, Ward, Kaplan: "Human mutation D157G in ferroportin leads to hepcidin-independent binding of Jak2 and ferroportin down-regulation." in: Blood, Vol. 115, Issue 14, pp. 2956-9, 2010

Tselepis, Ford, McKie, Vogel, Zoller, Simpson, Diaz Castro, Iqbal, Ward: "Characterization of the transition-metal-binding properties of hepcidin." in: The Biochemical journal, Vol. 427, Issue 2, pp. 289-96, 2010

Images

Product Details anti-Hepcidin Antibody Target Details Hepcidin Application Details Handling References for anti-Hepcidin antibody (ABIN394071) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Hepcidin Antimicrobial Peptide (HAMP) (AA 25-85) antibody (ABIN394071) Immunoperoxidase of monoclonal antibody to HAMP on formalin-fixed paraffin-embedded h...
Immunohistochemistry (IHC) image for anti-Hepcidin Antimicrobial Peptide (HAMP) (AA 25-85) antibody (ABIN394071) anti-Hepcidin Antimicrobial Peptide (HAMP) (AA 25-85) antibody (Image 2)
Western Blotting (WB) image for anti-Hepcidin Antimicrobial Peptide (HAMP) (AA 25-85) antibody (ABIN394071) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
ELISA image for anti-Hepcidin Antimicrobial Peptide (HAMP) (AA 25-85) antibody (ABIN394071) Detection limit for recombinant GST tagged HAMP is approximately 0.03ng/ml as a captu...