anti-Human AGT antibody for Immunoprecipitation

Recommended AGT Antibody (supplied by: Log in to see )

Antigen
Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) Antibodies
  • ANHU
  • SERPINA8
  • AI265500
  • AngI
  • AngII
  • Aogen
  • Serpina8
  • ANRT
  • Ang
  • PAT
  • wu:fb62f06
  • wu:fj87b02
  • zgc:111892
  • AGT
  • angt
  • ANGT
  • angiotensinogen
  • angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
  • AGT
  • Agt
  • agt
Epitope
AA 1-485
40
15
13
13
9
8
7
5
5
5
5
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
209
60
56
8
3
2
2
2
2
2
1
1
Host
Rabbit
156
87
2
1
1
Clonality
Monoclonal
Conjugate
This AGT antibody is un-conjugated
16
11
5
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC), Immunoprecipitation (IP), Western Blotting (WB)
154
107
69
48
22
20
14
14
7
4
4
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN1997925
$ 273.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2200881 IC IP WB Rabbit IgG Log in to see 13A156 0

General

Antigen Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) Antibodies
Epitope AA 1-485
(40), (15), (13), (13), (9), (8), (7), (5), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(209), (60), (56), (8), (3), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(156), (87), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AGT antibody is un-conjugated
(16), (11), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC), Immunoprecipitation (IP), Western Blotting (WB)
(154), (107), (69), (48), (22), (20), (14), (14), (7), (4), (4), (3), (2), (1), (1), (1), (1), (1)
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Product Details anti-AGT Antibody

Target Details AGT Application Details Handling Images
Specificity Human SerpinA8 / Angiotensinogen
No cross-reactivity in ELISA with
Human SerpinA1 / A1AT
Human SerpinA3 / SERPINA3 / AACT
Human SerpinA5 / Protein C inhibitor
Human SerpinD1 / HC2
Human SerpinF2 / SERPINF2
Human SerpinG1 / C1IN
Human SerpinI1 / Protease inhibitor 12
Human SerpinC1 /SERPINC1/ Antithrombin-III
No Cross-Reactivity Human
Characteristics This antibody was obtained from a rabbit immunized with purified, recombinant Human SerpinA8 / angiotensinogen (rh SerpinA8, ABIN2003702 NP_000020.1, Met 1-Ala 485,.
Sterility 0.2 μm filtered
Immunogen Recombinant Human SerpinA8 protein (ABIN2003702)
Clone 1
Isotype IgG

Target Details AGT

Product Details anti-AGT Antibody Application Details Handling Images back to top
Antigen
Alternative Name AGT (AGT Antibody Abstract)
Background Angiotensinogen, also known as AGT and SERPINA8, is a member of the serpin family. It is an α-2-globulin that is produced constitutively and released into the circulation mainly by the liver. Angiotensinogen is a essential component of the renin-angiotensin system (RAS) and a potent regulator of blood pressure. It is expressed by the liver and secreted in plasma. Angiotensinogen is cleaved into three chains: Angiotensin-1 (Ang I), Angiotensin-2 (Ang II), and Angiotensin-3 (Ang III). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2. Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system. Defects in AGT are associated with susceptibility to essential hypertension and renal tubular dysgenesis (RTD).
NCBI Accession NP_000020.1
Pathways JAK-STAT Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Monocarboxylic Acid Catabolic Process, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-AGT Antibody Target Details AGT Handling Images back to top
Application Notes WB: 2-5 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human AGT. The detection limit for Human AGT is 0.0195 ng/well.
ICC/IF: 10-25 μg/mL
IP: 4-6 μg/mg of lysate
Restrictions For Research Use only

Handling

Product Details anti-AGT Antibody Target Details AGT Application Details Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-AGT Antibody Target Details AGT Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) (AA 1-485) antibody (ABIN1997925) Immunofluorescence staining of Human AGT in Hela or SKBR3 cells. Cells were fixed wit...
Western Blotting (WB) image for anti-Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) (AA 1-485) antibody (ABIN1997925) anti-Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) (AA 1-485) antibody (Image 2)