anti-Mouse (Murine) Leptin antibody for ELISA

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Reactivity
Mouse (Murine)
442
178
157
26
9
6
6
5
5
5
3
3
3
2
2
1
1
1
1
1
Host
Rabbit
387
226
21
10
4
1
1
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
70
30
19
11
10
9
9
6
6
6
6
6
6
6
6
6
6
5
3
3
3
3
3
3
3
3
3
2
2
1
1
Application
ELISA, Western Blotting (WB)
451
412
148
55
54
44
39
30
29
28
26
17
13
9
5
4
4
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN465757
$ 493.17
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
15.755764 ABIN1169154 IP ELISA WB Biotin Rabbit Log in to see Polyclonal 0
12.755764 ABIN5706824 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
12.755764 ABIN5706823 ELISA WB Rabbit Log in to see Polyclonal 0
12.755764 ABIN2145804 Inhibition ELISA WB Rabbit Log in to see Polyclonal 0
12.755764 ABIN288442 ELISA WB Biotin Rabbit Log in to see Polyclonal 0
10 ABIN3201294 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 22-167 Log in to see 0
8.5 ABIN3043294 ELISA WB Rabbit IgG AA 74-109, Middle Region Log in to see Polyclonal 4
8.5 ABIN5692874 ELISA WB Rabbit AA 22-167 Log in to see Polyclonal 1
7 ABIN214037 ELISA IHC IHC (p) WB Rabbit Log in to see Polyclonal 0
7 ABIN465756 ELISA WB Biotin Rabbit Log in to see Polyclonal 0
7 ABIN205695 ELISA IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
7 ABIN5547256 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
7 ABIN2421035 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
5.5 ABIN5692873 ELISA IHC (p) WB Rabbit AA 22-167 Log in to see Polyclonal 1
4 ABIN2469665 IHC ELISA WB Rabbit Log in to see Polyclonal 0
4 ABIN1736401 IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal 0
4 ABIN2475319 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN108522 ELISA WB Rabbit Log in to see Polyclonal 6
1 ABIN2884678 IHC ELISA Rabbit IgG AA 36-85 Log in to see Polyclonal 0
1 ABIN2612691 ELISA WB Rabbit Log in to see Polyclonal 0

General

Antigen Leptin (LEP) Antibodies
Reactivity Mouse (Murine)
(442), (178), (157), (26), (9), (6), (6), (5), (5), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(387), (226), (21), (10), (4), (1), (1)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(70), (30), (19), (11), (10), (9), (9), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1)
Application ELISA, Western Blotting (WB)
(451), (412), (148), (55), (54), (44), (39), (30), (29), (28), (26), (17), (13), (9), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling Images
Specificity Mouse Leptin
Purification Immunoaffinity purified
Immunogen E.coli derived recombinant Murine Leptin.

Type of Immunogen: Cell extract
Plasmids, Primers & others

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Leptin (LEP Antibody Abstract)
Background Name/Gene ID: LEP

Synonyms: LEP, Leptin, LEPD, Obese protein, Obese, mouse, homolog of, Obesity factor, OBS, OB
Gene ID 3952
UniProt P41159
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
Application Notes Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained 4 % PFA, paraffin-embedded sections of Trichuris muris infected murine tissue. The primary antibody was incubated at 0.20 μg/mL overnight at 4 °C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100  mM citric acid was used. Information and photo are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary. ELISA: To detect mLeptin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine Leptin (LS-C104759) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mLeptin. Western Blot: To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLeptin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Comment

Target Species of Antibody: Mouse

Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitute with 50 μL sterile ddH2O.
Concentration Lot specific
Buffer Lyophilized from PBS,  pH 7.2
Handling Advice Avoid freeze/thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling back to top
Supplier Images
 image for anti-Leptin (LEP) antibody (ABIN465757) anti-Leptin (LEP) antibody
 image for anti-Leptin (LEP) antibody (ABIN465757) anti-Leptin (LEP) antibody (Image 2)
 image for anti-Leptin (LEP) antibody (ABIN465757) anti-Leptin (LEP) antibody (Image 3)
 image for anti-Leptin (LEP) antibody (ABIN465757) anti-Leptin (LEP) antibody (Image 4)
 image for anti-Leptin (LEP) antibody (ABIN465757) anti-Leptin (LEP) antibody (Image 5)