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|Antigen||Leptin (LEP) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This Leptin antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraformaldehyde) (IHC (pfa)), ELISA, Western Blotting (WB)
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Product Details anti-Leptin AntibodyTarget Details Leptin Application Details Handling Images
E.coli derived recombinant Murine Leptin.
Type of Immunogen: Cell extract
Target Details LeptinProduct Details anti-Leptin Antibody Application Details Handling Images back to top
|Alternative Name||Leptin (LEP Antibody Abstract)|
Name/Gene ID: LEP
Synonyms: LEP, Leptin, LEPD, Obese protein, Obese, mouse, homolog of, Obesity factor, OBS, OB
|Pathways||JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process|
Application DetailsProduct Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
Approved: ELISA, IHC-Pfa (0.2 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: This antibody stained 4 % PFA, paraffin-embedded sections of Trichuris muris infected murine tissue. The primary antibody was incubated at 0.20 μg/mL overnight at 4 °C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100 mM citric acid was used. Information and photo are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary. ELISA: To detect mLeptin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine Leptin (LS-C104759) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mLeptin. Western Blot: To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLeptin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
|Restrictions||For Research Use only|
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|Reconstitution||Reconstitute with 50 μL sterile ddH2O.|
|Buffer||Lyophilized from PBS, pH 7.2|
|Handling Advice||Avoid freeze/thaw cycles.|
|Storage||4 °C,-20 °C|
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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