anti-Primate Serotonin Receptor 2B antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Serotonin Receptor 2B Antibody (supplied by: Log in to see )

Antigen
Serotonin Receptor 2B (HTR2B) Antibodies
  • 5-HT2B
  • 5HT2B
  • 5htr2b
  • HTR2B
  • 5ht2b
  • zgc:194094
  • zgc:194119
  • AJ012488
  • AV377389
  • 5-HT(2B)
  • 5-hydroxytryptamine receptor 2B
  • 5-hydroxytryptamine (serotonin) receptor 2B, G protein-coupled
  • 5-hydroxytryptamine (serotonin) receptor 2B
  • HTR2B
  • htr2b
  • Htr2b
Epitope
2nd Extracellular Domain
12
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey, Primate
106
25
23
2
2
2
2
2
1
Host
Rabbit
104
4
Clonality
Polyclonal
Conjugate
This Serotonin Receptor 2B antibody is un-conjugated
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
61
34
28
28
23
22
7
5
4
1
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Catalog No. ABIN270524
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General

Antigen Serotonin Receptor 2B (HTR2B) Antibodies
Epitope 2nd Extracellular Domain
(12), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey, Primate
(106), (25), (23), (2), (2), (2), (2), (2), (1)
Host Rabbit
(104), (4)
Clonality
Polyclonal
Conjugate This Serotonin Receptor 2B antibody is un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(61), (34), (28), (28), (23), (22), (7), (5), (4), (1)
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Product Details anti-Serotonin Receptor 2B Antibody

Target Details Serotonin Receptor 2B Application Details Handling Images
Specificity Human 5HT2B Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Purification Immunogen affinity purified
Immunogen Synthetic 15 amino acid peptide from 2nd extracellular domain of human 5HT2B Receptor.
Plasmids, Primers & others

Target Details Serotonin Receptor 2B

Product Details anti-Serotonin Receptor 2B Antibody Application Details Handling Images back to top
Antigen
Alternative Name 5-HT2B (HTR2B Antibody Abstract)
Background Gene Symbol: HTR2B
Gene ID 3357
UniProt P41595
Pathways JAK-STAT Signaling, Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Carbohydrate Metabolic Process

Application Details

Product Details anti-Serotonin Receptor 2B Antibody Target Details Serotonin Receptor 2B Handling Images back to top
Application Notes Immunohistochemistry-Paraffin 4 - 6 μg/mLICC: Does not work.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for 5HT2B Receptor Antibody (NLS1111)Immunohistochemistry
1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
. Make 4 um sections and place on pre-cleaned and charged microscope slides.
. Heat in a tissue-drying oven for 45 minutes at 60 degrees Celcius.
. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each at RT.
. Rehydrate the tissues by washing the slides in 3 changes of 100 % alcohol for 3 minutes each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 3 minutes each at RT.
. Wash the slides in 1 change of 80 % alcohol for 3 minutes at RT.
. Rinse the slides in gentle running distilled water for 5 minutes at RT.
. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.
0) at 99-100 degrees Celciusfor 20 minutes.
. Remove the slides from the heat and let stand in buffer at RT for 20 minutes.
. Rinse the slides in 1X TBS-T for 1 minute at RT.**Do not allow the tissues to dry at any time during the staining procedure**
. Begin the immunostaining by applying a universal protein block for 20 minutes at RT.
. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase steptavidin for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase chromagen substrate for 30 minutes at RT.
. Rinse the slide in distilled water for 1 minute at RT.**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**
. Dehydrate the tissue by washing the slides in 2 changes of 80 % alcohol for 1 minute each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of 100 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of xylene for 1 minute each at RT.
. Apply cover slip.
Restrictions For Research Use only

Handling

Product Details anti-Serotonin Receptor 2B Antibody Target Details Serotonin Receptor 2B Application Details Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Images

Product Details anti-Serotonin Receptor 2B Antibody Target Details Serotonin Receptor 2B Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Serotonin Receptor 2B (HTR2B) (2nd Extracellular Domain) antibody (ABIN270524) Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1111] - Human Lung, Small Cell Car...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Serotonin Receptor 2B (HTR2B) (2nd Extracellular Domain) antibody (ABIN270524) Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1111] - Analysis of anti-5HT2B Rec...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Serotonin Receptor 2B (HTR2B) (2nd Extracellular Domain) antibody (ABIN270524) Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1111] - Human Brain, Glioblastoma....