PAI1 ELISA Kit
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- Target See all PAI1 (SERPINE1) ELISA Kits
- PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))
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Reactivity
- Human, Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 80-20.000 pg/mL
- Minimum Detection Limit
- 80 pg/mL
- Application
- ELISA
- Purpose
- Mouse PAI-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Sample Type
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificity
- This ELISA antibody pair detects mouse and human PAI-1.
- Sensitivity
- 80 pg/mL
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our top product SERPINE1 ELISA Kit
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- Application Notes
- Recommended Dilution for serum and plasma samples20 fold
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Assay Procedure
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Expiry Date
- 6 months
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- Target See all PAI1 (SERPINE1) ELISA Kits
- PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))
- Alternative Name
- PAI-1 (SERPINE1 Products)
- Synonyms
- PAI ELISA Kit, PAI-1 ELISA Kit, PAI1 ELISA Kit, PLANH1 ELISA Kit, SERPINE1 ELISA Kit, si:ch211-138a11.1 ELISA Kit, Planh1 ELISA Kit, PAI1A ELISA Kit, Pai1 ELISA Kit, Pai1aa ELISA Kit, Planh ELISA Kit, RATPAI1A ELISA Kit, serpin family E member 1 ELISA Kit, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 L homeolog ELISA Kit, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade E, member 1 ELISA Kit, SERPINE1 ELISA Kit, serpine1.L ELISA Kit, serpine1 ELISA Kit, Serpine1 ELISA Kit
- Background
- Plasminogen activator inhibitor 1 (PAI) (PAI-1) (Endothelial plasminogen activator inhibitor) (Serpin E1)
- UniProt
- P22777
- Pathways
- p53 Signaling, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration
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