STAT3 ELISA Kit

Catalog No. ABIN2866294

Product Details STAT3 ELISA Kit

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: DNA-Binding ELISA

Application: ELISA

Purpose: DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.

Brand: TransAM®

Sample Type: Cell Extracts, Tissue Samples

Analytical Method: Quantitative

Specificity: TransAM STAT3 Kits are tested for sensitivity in detecting STAT activation.

Characteristics: Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied.

Components: One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Nuclear extracts prepared from HepG2 cells that are either unstimulated or stimulated with IL-6 are diluted to 0.625 μg/well and assayed using the TransAM STAT3 Kit. The ratio of the signals from the stimulated over unstimulated cells must be above 4. Lot No.04114017 was developed for 10 minutes. It gave a ratio of 4.8 at 5ug/well (Figure 1). This ratio may vary depending on the basal level of STAT activation in a given cell type and the stimulation used. TransAM STAT3 Kits are also tested for specificity in detecting STAT activation. TransAM STAT assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated STAT consensus binding site (Figure 2). At 20X excess, the wild-type oligonucleotide prevents STAT binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has no effect on STAT binding.

Assay Time: 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Store the cell extract at -80°C. Other kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the antibody, oligonucleotides, DTT, and Protease Inhibitor Cocktail that should be kept at -20°C. This product is guaranteed for 6 months from date of receipt.

Expiry Date: 6 months

Target Details for STAT3

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Alternative Name: Stat3 (STAT3 Products)

Synonyms: 1110034C02Rik ELISA Kit, AW109958 ELISA Kit, Aprf ELISA Kit, APRF ELISA Kit, HIES ELISA Kit, Xstat3 ELISA Kit, aprf ELISA Kit, hies ELISA Kit, stat3 ELISA Kit, wu:fc15d02 ELISA Kit, wu:fl59g06 ELISA Kit, z-Stat3 ELISA Kit, signal transducer and activator of transcription 3 ELISA Kit, signal transduction and activation of transcription 3 ELISA Kit, signal transducer and activator of transcription 3, gene 1 L homeolog ELISA Kit, signal transducer and activator of transcription 3 (acute-phase response factor) ELISA Kit, STAT3 ELISA Kit, stat3 ELISA Kit, Stat3 ELISA Kit, stat3.1.L ELISA Kit

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor