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|Antigen||Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies|
|Conjugate||This EIF4EBP1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA)
|2 references available|
|Supplier||Log in to see|
Product Details anti-EIF4EBP1 AntibodyTarget Details EIF4EBP1 Application Details Handling References for anti-EIF4EBP1 antibody (ABIN358355) Images
|Specificity||This antibody detects 4E-BP1/EIF4EBP1 pThr36. Predicted to cross react with Mouse (100 % Antigen Homology).|
|Purification||Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T36 of human EIF4EBP1.|
Target Details EIF4EBP1Product Details anti-EIF4EBP1 Antibody Application Details Handling References for anti-EIF4EBP1 antibody (ABIN358355) Images back to top
|Alternative Name||EIF4EBP1 / 4E-BP1 (EIF4EBP1 Antibody Abstract)|
|Background||EIF4EBP1 is a member of a family of translation repressor proteins. This protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. It is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.Synonyms: Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-1, PHAS-I, PHAS1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, eIF4E-binding protein 1|
|Molecular Weight||12449 Da|
|Gene ID||1978, 9606|
|Pathways||MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size|
Application DetailsProduct Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Handling References for anti-EIF4EBP1 antibody (ABIN358355) Images back to top
ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details References for anti-EIF4EBP1 antibody (ABIN358355) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-EIF4EBP1 antibody (ABIN358355)Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling Images back to top
Fonseca, Smith, Lee, MacKintosh, Proud: "PRAS40 is a target for mammalian target of rapamycin complex 1 and is required for signaling downstream of this complex." in: The Journal of biological chemistry, Vol. 282, Issue 34, pp. 24514-24, 2007
Armengol, Rojo, Castellví, Iglesias, Cuatrecasas, Pons, Baselga, Ramón y Cajal: "4E-binding protein 1: a key molecular "funnel factor" in human cancer with clinical implications." in: Cancer research, Vol. 67, Issue 16, pp. 7551-5, 2007
ImagesProduct Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling References for anti-EIF4EBP1 antibody (ABIN358355) back to top