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|Antigen||Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies|
|Conjugate||This MAP3K5 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-MAP3K5 AntibodyTarget Details MAP3K5 Application Details Handling Images
|Specificity||This antibody reacts to ASK1 (MEKK5/MAP3K5).|
|Purification||Affinity chromatography on Protein A|
|Immunogen||KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S83 of human MAP3K5.|
Target Details MAP3K5Product Details anti-MAP3K5 Antibody Application Details Handling Images back to top
|Alternative Name||MEKK5 / ASK1 (MAP3K5 Antibody Abstract)|
|Background||Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5|
|Gene ID||4217, 9606|
|Pathways||MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded protein response|
Application DetailsProduct Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling Images back to top
ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|