|Antigen||Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAP4K3 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Product Details anti-MAP4K3 AntibodyTarget Details MAP4K3 Application Details Handling Images
|Specificity||GLK antibody detects endogenous levels of GLK protein.(region surrounding Gly4)|
|Purification||Affinity Chromatography using epitope-specific immunogen.|
Target Details MAP4K3Product Details anti-MAP4K3 Antibody Application Details Handling Images back to top
|Alternative Name||MAP4K3 (MAP4K3 Antibody Abstract)|
|Background||Several mammalian kinases have been identified which exhibit sequence similarity to the Saccharomyces cerevisiae serine/threonine kinase STE20. STE20 is involved in relaying signals from G-protein coupled receptors to cytosolic MAP kinase cascades, and it lies upstream of a MAP kinase kinase kinase. Mammalian STE20-like kinases include GLK, KHS, NIK, YSK1, HPK1, Krs-1, Krs-2 and human GC kinase. GLK (for GC-like kinase) is an 885 amino acid protein that shares a high degree of homology with GC kinase and HPK1. Like many of the STE20-like kinases, GLK specifically activates the JNK pathway. Epistasis studies with dominant negative mutants of MEKK1 suggest that GLK functions upstream of MEKK1 in the JNK signaling pathway.Synonyms: Germinal center kinase-related protein kinase, MAPK, MAPK/ERK kinase kinase kinase 3, MEKKK 3, Mitogen-activated protein kinase kinase kinase kinase 3, RAB8IPL1|
|Molecular Weight||approx. 101 kDa|
Application DetailsProduct Details anti-MAP4K3 Antibody Target Details MAP4K3 Handling Images back to top
ELISA: 1/20000approx. 1/40000. Western Blot: 1/500approx. 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP4K3 Antibody Target Details MAP4K3 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-MAP4K3 Antibody Target Details MAP4K3 Application Details Handling back to top