anti-Mouse (Murine) PPARG antibody for Immunocytochemistry

Recommended PPARG Antibody (supplied by: Log in to see )

Antigen
Peroxisome Proliferator-Activated Receptor gamma (PPARG) Antibodies
  • PPAR gamma
  • PPARg2
  • PPARG
  • Nr1c3
  • PPAR-gamma
  • PPAR-gamma2
  • PPARgamma
  • PPARgamma2
  • NR1C3
  • CIMT1
  • GLM1
  • PPARG1
  • PPARG2
  • xPPAR-gamma
  • PPAR-GAMMA
  • PPARGAMMA
  • peroxisome proliferator-activated receptor gamma
  • peroxisome proliferator activated receptor gamma
  • peroxisome proliferator activated receptor gamma L homeolog
  • PPARG
  • Pparg
  • pparg.L
Reactivity
Human, Mouse (Murine), Rat (Rattus)
297
160
126
28
23
14
12
11
10
9
6
5
5
4
4
4
4
4
3
2
2
2
1
1
Host
Rabbit
278
43
5
4
Clonality
Polyclonal
Conjugate
This PPARG antibody is un-conjugated
20
15
9
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
272
128
81
44
39
30
28
21
18
14
9
6
4
3
2
2
Supplier
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Catalog No. ABIN6264371
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN6254905 ELISA ICC IF IHC WB Rabbit IgG N-Term Log in to see Polyclonal 1
1 ABIN6256109 ELISA ICC IF IHC WB Rabbit IgG pSer112 Log in to see Polyclonal 0
1 ABIN1173890 ICC IHC WB Rabbit IgG AA 149-273 Log in to see Polyclonal 0
1 ABIN1869907 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN4368897 ICC WB Mouse AA 254-478, C-Term Log in to see 0

General

Antigen Peroxisome Proliferator-Activated Receptor gamma (PPARG) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(297), (160), (126), (28), (23), (14), (12), (11), (10), (9), (6), (5), (5), (4), (4), (4), (4), (4), (3), (2), (2), (2), (1), (1)
Host Rabbit
(278), (43), (5), (4)
Clonality
Polyclonal
Conjugate This PPARG antibody is un-conjugated
(20), (15), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(272), (128), (81), (44), (39), (30), (28), (21), (18), (14), (9), (6), (4), (3), (2), (2)
Pubmed 2 references available
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Product Details anti-PPARG Antibody

Target Details PPARG Application Details Handling References for anti-PPARG antibody (ABIN6264371) Images
Specificity PPAR-gamma Antibody detects endogenous levels of total PPAR-gamma
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Immunogen A synthesized peptide derived from human PPAR-gamma
Isotype IgG
Plasmids, Primers & others

Target Details PPARG

Product Details anti-PPARG Antibody Application Details Handling References for anti-PPARG antibody (ABIN6264371) Images back to top
Antigen
Alternative Name PPAR-gamma (PPARG Antibody Abstract)
Background

Description: Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (By similarity).

Gene: PPARG

Molecular Weight 57kDa
Gene ID 5468
UniProt P37231
Pathways MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-PPARG Antibody Target Details PPARG Handling References for anti-PPARG antibody (ABIN6264371) Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-PPARG Antibody Target Details PPARG Application Details References for anti-PPARG antibody (ABIN6264371) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

References for anti-PPARG antibody (ABIN6264371)

Product Details anti-PPARG Antibody Target Details PPARG Application Details Handling Images back to top
Product cited in:

Song, Zhou, Peng, Tao, Yang, Xu, Peng, Ren, Xiang, Wei: "GPR120 promotes adipogenesis through intracellular calcium and extracellular signal-regulated kinase 1/2 signal pathway." in: Molecular and cellular endocrinology, Vol. 434, pp. 1-13, 2017

Hu, Luo, Peng, Song, Zhou, Wei, Peng, Jiang: "Molecular cloning, expression pattern analysis of porcine Rb1 gene and its regulatory roles during primary dedifferentiated fat cells adipogenic differentiation." in: General and comparative endocrinology, Vol. 214, pp. 77-86, 2015

Images

Product Details anti-PPARG Antibody Target Details PPARG Application Details Handling References for anti-PPARG antibody (ABIN6264371) back to top
Supplier Images
Western Blotting (WB) image for anti-Peroxisome Proliferator-Activated Receptor gamma (PPARG) antibody (ABIN6264371) Western blot analysis of PPAR-gamma expression in HuvEc whole cell lysates,The lane o...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Peroxisome Proliferator-Activated Receptor gamma (PPARG) antibody (ABIN6264371) ABIN6269231 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized...
Immunohistochemistry (IHC) image for anti-Peroxisome Proliferator-Activated Receptor gamma (PPARG) antibody (ABIN6264371) ABIN6269231 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehy...
Western Blotting (WB) image for anti-Peroxisome Proliferator-Activated Receptor gamma (PPARG) antibody (ABIN6264371) Western blot analysis of extracts from various samples, using PPAR-gamma Antibody. La...