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|Antigen||Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) Antibodies|
|Reactivity||Chicken, Cow (Bovine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine) Alternatives|
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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|Specificity||Recognizes 55 Kda Aromatase band in human, mouse, rat, bovine, porcine, pig, sheep, rabbit, horse and chicken. Additional 35 kD band (believed to be the cleavage product of Aromatase) can also be detected in Jurkat cells.|
Synthetic peptide surrounding amino acid 385 of human Aromatase.
Type of Immunogen: Synthetic peptide
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|Alternative Name||CYP19 / Aromatase (CYP19A1 Antibody Abstract)|
Name/Gene ID: CYP19A1
Family: Cytochrome P450
Synonyms: CYP19A1, ARO1, CYAR, CPV1, CYPXIX, Cytochrome P-450AROM, Cytochrome P450 19A1, CYP19, Estrogen synthase, Estrogen synthetase, P-450AROM, ARO, Aromatase
|Pathways||Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis|
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Approved: IHC, IHC-P (10 μg/mL), WB (0.5 - 4 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.
Target Species of Antibody: Human
|Restrictions||For Research Use only|
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|Buffer||PBS, 30 % glycerol, 0.5 % BSA, 0.5 mM EDTA, 0.03 % ProClin|
|Precaution of Use||This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,-80 °C|
Short term: -20°C
Long term: -70°C
Avoid freeze-thaw cycles.
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