anti-Mouse (Murine) ROCK1 antibody for ELISA

Recommended ROCK1 Antibody (supplied by: Log in to see )

Antigen
rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) Antibodies
  • LOC100229794
  • CG9774
  • DROK
  • DRhk
  • DRok
  • Dmel\\CG9774
  • Drok
  • ROCK
  • ROCK-1
  • ROCK1
  • ROK
  • ROKalpha/beta
  • Rhk
  • RhoK
  • Rock
  • Rok
  • dROK
  • dRok
  • drok
  • 1110055K06Rik
  • Rock-I
  • P160Rock
  • ROCK-I
  • P160ROCK
  • p160ROCK
  • Rho associated coiled-coil containing protein kinase 1
  • Rho kinase
  • Rho-associated coiled-coil containing protein kinase 1
  • Rho-associated, coiled-coil containing protein kinase 1
  • ROCK1
  • Rok
  • Rock1
  • rock1
Epitope
pTyr913
38
27
24
24
18
15
5
5
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
187
147
137
22
18
17
8
5
4
2
1
Host
Rabbit
125
64
Clonality
Polyclonal
Conjugate
This ROCK1 antibody is un-conjugated
7
6
6
6
5
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
Application
ELISA, Western Blotting (WB)
147
42
38
36
35
30
26
16
4
2
2
1
1
Supplier
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Catalog No. ABIN6267814
$ 450.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN6267816 ELISA WB Rabbit IgG pSer282 Log in to see Polyclonal 0
1 ABIN3186829 ELISA ICC IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0
1 ABIN6268230 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6268232 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6269447 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN3186828 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 0
1 ABIN5958486 ELISA IF IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1031544 ELISA WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN1453860 IHC ELISA WB Rabbit IgG AA 262-311 Log in to see Polyclonal 0
1 ABIN4350897 ELISA ICC IF WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN2885883 IHC ELISA WB Rabbit IgG AA 262-311 Log in to see Polyclonal 0
1 ABIN2751305 ELISA IHC IP WB FITC Rabbit IgG AA 1300-1360 Log in to see Polyclonal 0
1 ABIN2751304 ELISA IHC IP WB Rabbit IgG AA 1300-1360 Log in to see Polyclonal 0
1 ABIN6106070 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0
1 ABIN6103327 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 0
1 ABIN5699552 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6044074 ELISA IHC Rabbit IgG Log in to see Polyclonal 0
1 ABIN6044075 ELISA IHC Rabbit IgG Log in to see Polyclonal 0
1 ABIN2924472 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2428675 IHC ELISA Rabbit IgG Log in to see Polyclonal 0

General

Antigen rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) Antibodies
Epitope pTyr913
(38), (27), (24), (24), (18), (15), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(187), (147), (137), (22), (18), (17), (8), (5), (4), (2), (1)
Host Rabbit
(125), (64)
Clonality
Polyclonal
Conjugate This ROCK1 antibody is un-conjugated
(7), (6), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application ELISA, Western Blotting (WB)
(147), (42), (38), (36), (35), (30), (26), (16), (4), (2), (2), (1), (1)
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Product Details anti-ROCK1 Antibody

Target Details ROCK1 Application Details Handling Images
Specificity Phospho-ROCK1(Tyr913) Antibody detects endogenous levels of ROCK1 only when phosphorylated at Tyr913
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human ROCK1 around the phosphorylation site of Tyr913
Isotype IgG
Plasmids, Primers & others

Target Details ROCK1

Product Details anti-ROCK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ROCK1 (ROCK1 Antibody Abstract)
Background

Description: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.

Gene: ROCK1

Molecular Weight 160kDa
Gene ID 6093
UniProt Q13464
Pathways Microtubule Dynamics, WNT Signaling, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling

Application Details

Product Details anti-ROCK1 Antibody Target Details ROCK1 Handling Images back to top
Application Notes WB 1:500-1:2000
Restrictions For Research Use only

Handling

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (pTyr913) antibody (ABIN6267814) Western blot analysis of Phospho-ROCK1(Tyr913) in lysates of cos-7, using Phospho-ROC...