anti-Rat (Rattus) Insulin Receptor antibody for Immunoprecipitation

Recommended Insulin Receptor Antibody (supplied by: Log in to see )

Antigen
Insulin Receptor (INSR) Antibodies
  • CD220
  • HHF5
  • 4932439J01Rik
  • D630014A15Rik
  • IR
  • IR-A
  • IR-B
  • 18402
  • CG18402
  • DIHR
  • DILR
  • DIR
  • DIRH
  • DIRbeta
  • DInR
  • DInr
  • Dir-a
  • Dir-b
  • Dmel\\CG18402
  • INR
  • INS
  • Inr
  • Inr-alpha
  • Inr-beta
  • InsR
  • dINR
  • dIR
  • dIRH
  • dInR
  • dInr
  • dInsR
  • dinr
  • dir
  • er10
  • inr
  • insulin/insulin-like growth factor receptor
  • l(3)05545
  • l(3)93Dj
  • l(3)er10
  • lnR
  • ir-A
  • CTK-1
  • ir
  • INSR
  • NV14476
  • cd220
  • hhf5
  • insulin receptor
  • Insulin-like receptor
  • insulin receptor L homeolog
  • INSR
  • Insr
  • InR
  • LOC100122567
  • LOC100451802
  • insr.L
Epitope
AA 1006-1144
40
34
22
22
17
14
12
10
9
8
8
7
6
6
6
5
5
5
5
5
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
321
128
96
23
23
21
20
17
17
9
5
5
3
1
1
1
1
1
Host
Mouse
252
93
8
1
1
Clonality
Monoclonal
Conjugate
This Insulin Receptor antibody is un-conjugated
25
18
12
10
10
9
6
5
5
5
4
4
4
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
215
138
75
47
45
45
17
13
13
11
11
8
7
4
3
2
2
2
1
Options
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Pubmed
5 references available

Available images

Western Blotting (WB) image for anti-Insulin Receptor (INSR) (AA 1006-1144) antibody (ABIN967753)
Immunofluorescence (IF) image for anti-Insulin Receptor (INSR) (AA 1006-1144) antibody (ABIN967753)
image for anti-Insulin Receptor (INSR) (AA 1006-1144) antibody (ABIN967753)
Western Blotting (WB) image for anti-Insulin Receptor (INSR) (AA 1006-1144) antibody (ABIN967753)
Catalog No. ABIN967753
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2704927 IP WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN967752 IF IP WB Mouse IgG1 AA 1006-1144 Log in to see 46-CD220 5
1 ABIN386229 IHC (p) IP ELISA WB Mouse IgG1 Log in to see CT-3 0
1 ABIN5663544 ICC IP WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN1495036 IP Magnetic Particles Rabbit N-Term Log in to see Polyclonal 0
1 ABIN1495033 IHC (p) IP WB Sepharose Rabbit N-Term Log in to see Polyclonal 0
1 ABIN603332 IP WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN2610257 IP WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN2750326 ELISA IHC IP WB FITC Rabbit IgG AA 950-1000, C-Term Log in to see Polyclonal 0
1 ABIN2750325 IP ELISA WB FITC Rabbit IgG AA 900-950, N-Term Log in to see Polyclonal 0
1 ABIN2750323 IP ELISA WB Rabbit IgG AA 900-950, N-Term Log in to see Polyclonal 0
1 ABIN2750324 ELISA IHC IP WB Rabbit IgG AA 950-1000, C-Term Log in to see Polyclonal 0
1 ABIN574496 ELISA IHC (p) IP WB Mouse IgG1 Log in to see CT-3 0
1 ABIN5557676 ELISA IHC IP WB Biotin Rabbit C-Term Log in to see Polyclonal 0
1 ABIN5557675 ELISA IP WB Biotin Rabbit N-Term Log in to see Polyclonal 0

General

Antigen Insulin Receptor (INSR) Antibodies
Epitope AA 1006-1144
(40), (34), (22), (22), (17), (14), (12), (10), (9), (8), (8), (7), (6), (6), (6), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine), Rat (Rattus)
(321), (128), (96), (23), (23), (21), (20), (17), (17), (9), (5), (5), (3), (1), (1), (1), (1), (1)
Host Mouse
(252), (93), (8), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin Receptor antibody is un-conjugated
(25), (18), (12), (10), (10), (9), (6), (5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(215), (138), (75), (47), (45), (45), (17), (13), (13), (11), (11), (8), (7), (4), (3), (2), (2), (2), (1)
Pubmed 5 references available
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Product Details anti-Insulin Receptor Antibody

Target Details Insulin Receptor Application Details Handling References for anti-Insulin Receptor antibody (ABIN967753) Images
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Insulin pro-receptor beta subunit 1006-1144
Clone 46-CD220
Isotype IgG1

Target Details Insulin Receptor

Product Details anti-Insulin Receptor Antibody Application Details Handling References for anti-Insulin Receptor antibody (ABIN967753) Images back to top
Antigen
Alternative Name CD220 (INSR Antibody Abstract)
Background Insulin Receptor (IR) is a transmembrane receptor tyrosine kinase which, upon insulin binding, initiates a cascade of events, including autophosphorylation, phosphorylation of cellular protein substrates, glucose transport, and glycogen synthesis. IR is synthesized as a large glycosylated precursor that is cleaved upon maturation into a 130 kDa alpha-subunit with kinase activity and a 95 kDa beta-subunit. The active Insulin Receptor is a heterotetramer of homologus alpha and beta subunits joined by disulfide bonds. Among the major cytosolic substrates of the Insulin Receptor are IRS-1 and -2, beta-Adrenergic receptor, and pp15 (adipocyte lipid-binding protein, ALBP). Autophosphorylation of the IR recruits IRS-1 and -2 to the phosphotyrosines. Subsequently, the phosphorylated IRS-1 and -2 act as docking sites for other signaling proteins like PI3-Kinase, Shc, PTP1D, Nck, etc. In addition, the phosphatase LAR is tightly associated with the IR and LAR becomes activated after insulin stimulation dephosphorylating the IR and its substrates. Therefore, LAR provides a turn-off mechanism in insulin signaling. This antibody is routinely tested by western blot analysis.
Synonyms: Insulin Receptor beta
Molecular Weight 95 kDa
Pathways NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity

Application Details

Product Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Handling References for anti-Insulin Receptor antibody (ABIN967753) Images back to top
Comment

Related Products: ABIN967389
Restrictions For Research Use only

Handling

Product Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details References for anti-Insulin Receptor antibody (ABIN967753) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-Insulin Receptor antibody (ABIN967753)

Product Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details Handling Images back to top
Product cited in:

Shumay, Song, Wang, Malbon: "pp60Src mediates insulin-stimulated sequestration of the beta(2)-adrenergic receptor: insulin stimulates pp60Src phosphorylation and activation." in: Molecular biology of the cell, Vol. 13, Issue 11, pp. 3943-54, 2002

Zundel, Swiersz, Giaccia: "Caveolin 1-mediated regulation of receptor tyrosine kinase-associated phosphatidylinositol 3-kinase activity by ceramide." in: Molecular and cellular biology, Vol. 20, Issue 5, pp. 1507-14, 2000

Frattali, Treadway, Pessin: "Insulin/IGF-1 hybrid receptors: implications for the dominant-negative phenotype in syndromes of insulin resistance." in: Journal of cellular biochemistry, Vol. 48, Issue 1, pp. 43-50, 1992

Yip, Jack: "Insulin receptors are bivalent as demonstrated by photoaffinity labeling." in: The Journal of biological chemistry, Vol. 267, Issue 19, pp. 13131-4, 1992

Ebina, Ellis, Jarnagin, Edery, Graf, Clauser, Ou, Masiarz, Kan, Goldfine: "The human insulin receptor cDNA: the structural basis for hormone-activated transmembrane signalling." in: Cell, Vol. 40, Issue 4, pp. 747-58, 1985

Images

Product Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details Handling References for anti-Insulin Receptor antibody (ABIN967753) back to top
Supplier Images
Western Blotting (WB) image for anti-Insulin Receptor (INSR) (AA 1006-1144) antibody (ABIN967753) Western blot analysis of CD220 on a mouse liver lysate. Lane 1: 1:250, lane 2: 1:500,...
Immunofluorescence (IF) image for anti-Insulin Receptor (INSR) (AA 1006-1144) antibody (ABIN967753) Immunofluorescence staining of PFSK-1 cells (human neuroectodermal tumor line, ATCC C...
image for anti-Insulin Receptor (INSR) (AA 1006-1144) antibody (ABIN967753) anti-Insulin Receptor (INSR) (AA 1006-1144) antibody (Image 3)
Western Blotting (WB) image for anti-Insulin Receptor (INSR) (AA 1006-1144) antibody (ABIN967753) anti-Insulin Receptor (INSR) (AA 1006-1144) antibody (Image 4)