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|Antigen||Nerve Growth Factor Receptor (NGFR) Antibodies|
|Epitope||AA 1-160, N-Term Alternatives|
|Reactivity||Cat (Feline), Ferret, Human, Monkey, Rabbit Alternatives|
|Conjugate||This NGFR antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|3 references available|
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Product Details anti-NGFR AntibodyTarget Details NGFR Application Details Handling References for anti-NGFR antibody (ABIN180341) Images
This antibody recognizes CD271/NGFR, a transmembrane glycoprotein of the TNFR superfamily. The epitope is localized within amino acids 1-160.
Cellular Localization: Cell Surface, Cytoplasmic.
|Cross-Reactivity (Details)||Species reactivity (tested):Human, Monkey (Cebus apella, Macaca fascicularis, mulatta, and nemestrina), Baboon (Papio anubis and Cynocephalus), Cat, Rabbit, and Ferret.|
|Characteristics||Synonyms: TNFRSF16, Tumor necrosis factor receptor superfamily member 16|
|Purification||Protein-A affinity chromatography|
|Immunogen||NGFR (75kD) from A875 Melanoma Cells.|
Target Details NGFRProduct Details anti-NGFR Antibody Application Details Handling References for anti-NGFR antibody (ABIN180341) Images back to top
|Alternative Name||CD271 / NGFR (NGFR Antibody Abstract)|
|Background||CD271 / NGFR, also known as p75NGFR or p75NTR, is a 75 kDa low affinity receptor for the NGF (nerve growth factor), BDNF (brain-derived growth factor), and other neurotrophins, such as NT3 and NT4/5. Unlike other members of the tumor necrosis factor receptor superfamily of transmembrane proteins, CD271 has unique intracellular domain structure (lacks catalytic activity) and downstream signaling partners. Triggered by its ligands CD271 affects growth, differentiation, migration and death of the nervous system cells.Synonyms: TNFRSF16, Tumor necrosis factor receptor superfamily member 16|
|Molecular Weight||75 kDa|
|Research Area||Growth Factors, Receptors|
|Pathways||NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding|
Application DetailsProduct Details anti-NGFR Antibody Target Details NGFR Handling References for anti-NGFR antibody (ABIN180341) Images back to top
Flow Cytometry. Immunocytochemistry. Inhibits Binding of NGF to Melanoma Cells (see Ref.1, use antibody without azide: ABIN180340). Not suitable for Western Blotting andImmunohistochemistry. Recommended Positive Control: Melanoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-NGFR Antibody Target Details NGFR Application Details References for anti-NGFR antibody (ABIN180341) Images back to top
|Buffer||PBS, pH ~7.4, 15 mM Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store at 2-8 °C for up to one month or in aliquots at -20 °C for longer. Avoid repeated freezethaw cycles!
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-NGFR antibody (ABIN180341)Product Details anti-NGFR Antibody Target Details NGFR Application Details Handling Images back to top
|Product cited in:||
Alpers, Hudkins, Ferguson, Johnson, Schatteman, Bothwell: "Nerve growth factor receptor expression in fetal, mature, and diseased human kidneys." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 69, Issue 6, pp. 703-13, 1994
Schatteman, Gibbs, Lanahan, Claude, Bothwell: "Expression of NGF receptor in the developing and adult primate central nervous system." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 8, Issue 3, pp. 860-73, 1988
Marano, Dietzschold, Earley, Schatteman, Thompson, Grob, Ross, Bothwell, Atkinson, Koprowski: "Purification and amino terminal sequencing of human melanoma nerve growth factor receptor." in: Journal of neurochemistry, Vol. 48, Issue 1, pp. 225-32, 1987