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General |
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Antigen | Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) Antibodies |
Epitope | AA 195-211, N-Term Alternatives |
Reactivity | Goat, Human, Mouse (Murine), Rat (Rattus) Alternatives |
Host | Rabbit Alternatives |
Clonality | |
Conjugate | This TNFRSF1A antibody is un-conjugated Alternatives |
Application |
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Alternatives
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Supplier | Log in to see |
Product Details anti-TNFRSF1A AntibodyTarget Details TNFRSF1A Application Details Handling Images |
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Specificity | This antibody detects CD120a / TNFR1 (195-211). No cross reactivity with other proteins. |
Cross-Reactivity (Details) | Species reactivity (tested):Goat, human, mouse, rat |
Purification | Immunogen affinity purified |
Immunogen | A synthetic peptide corresponding to a sequence at the N-terminal of human TNFR1 .Remarks: Sequence id different to the related rat sequence by a single amino acid. |
Isotype | IgG |
Target Details TNFRSF1AProduct Details anti-TNFRSF1A Antibody Application Details Handling Images back to top |
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Antigen | |
Alternative Name | CD120a / TNFR1 (TNFRSF1A Antibody Abstract) |
Background | Tumor necrosis factor receptor 1(TNFR1), a potent cytokine, elicits a broad spectrum of biologic responses which are mediated by binding to a cell surface receptor. Its gene is located on 12p13.2. The coding region and the 3-prime untranslated region of TNFR1 are distributed over 10 exons. There are 2 different proteins that serve as major receptors for TNF-alpha, one associated with myeloid cells and one associated with epithelial cells. Additionally, TNFR1 associates with the MADD protein through a death domain-death domain interaction. MADD provides a physical link between TNFR1 and the induction of mitogen-activated protein (MAP) kinase (e.g., ERK2) activation and arachidonic acid release. TNFR1-induced apoptosis involves 2 sequential signaling complexes. Complex I, the initial plasma membrane-bound complex, consists of TNFR1, the adaptor TRADD, the kinase RIP1, and TRAF2 and rapidly signals activation of NF-kappa-B. In a second step, TRADD and RIP1 associate with FADD and caspase-8, forming a cytoplasmic complex, complex II.Synonyms: TNF-R1, TNF-RI, TNFR-I, Tnfrsf1a, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor type I, p55, p60 |
Gene ID | 7132 |
NCBI Accession | NP_001056 |
UniProt | P19438 |
Pathways | NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Hepatitis C |
Application DetailsProduct Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Handling Images back to top |
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Application Notes | Optimal working dilution should be determined by the investigator. |
Restrictions | For Research Use only |
HandlingProduct Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Application Details Images back to top |
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Reconstitution | 0.2 mL of distilled water will yield a concentration of 500 μg/mL. |
Buffer | 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3 |
Preservative | Sodium azide, Thimerosal (Merthiolate) |
Precaution of Use | This product contains Sodium azide and Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only. |
Handling Advice | Avoid repeated freezing and thawing. |
Storage | 4 °C/-20 °C |
Storage Comment | Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. |