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|Antigen||Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) Antibodies|
|Epitope||Extracellular Domain Alternatives|
|Reactivity||Human, Primate Alternatives|
|Conjugate||This TNFRSF1A antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
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Product Details anti-TNFRSF1A AntibodyTarget Details TNFRSF1A Application Details Handling Images
|Specificity||The extracellular domain of the cell bound human TNFRp55. In general, this antibody also detects primate sTNFRp55. The antibody does not recognize human sTNFRp75. In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the TNFRp55 found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid|
Recombinant human sTNFRp55 expressed in Chinese Hamster Cells.
Type of Immunogen: Recombinant protein
|Plasmids, Primers & others|
Target Details TNFRSF1AProduct Details anti-TNFRSF1A Antibody Application Details Handling Images back to top
|Alternative Name||TNFRSF1A / TNFR1 (TNFRSF1A Antibody Abstract)|
Name/Gene ID: TNFRSF1A
Family: TNF Receptor
Synonyms: TNFRSF1A, CD120a, CD120a antigen, MS5, p60, TBP1, TNF-R55, TNFAR, TNFR1, p55-R, TNF-R, TNF-R-I, TNF-R1, TNF-RI, Tnfr, TNFR-I, TNFR1-d2, TNFR60, FPF, p55, Tbpi, TNFR55
|Pathways||NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Hepatitis C|
Application DetailsProduct Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Handling Images back to top
Approved: ELISA (1:1000 - 1:2000), IP, RIA, WB
Usage: This antiserum against Human TNF p55 Receptor (sTNFRp55) has been tested for use in ELISA, radioimmunoassays, immunoblotting and immunoprecipitation. Reactivity in other immunoassays is unknown. This product has been assayed for the ability to immunoprecipitate antigen. A dilution range of 1:400 to 1:800 is suggested for this immunoassay. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L] (catalog no. LS-C60884) and TMB as a substrate. A working dilution range of 1:200 to 1:400 is suggested for this application. This product has been assayed by ELISA against soluble (extracellular) sTNFRp55 using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (catalog no. LS-C60884) and ABTS as a substrate for 30 minutes at room temperature. A working dilution range of 1:1000 to 1:2000 is suggested for this product. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This product has been assayed by radioimmunoassay against antigen. A dilution of 1:8000 is suggested for this immunoassay.
Target Species of Antibody: Human
|Restrictions||For Research Use only|
HandlingProduct Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Application Details Images back to top
|Buffer||0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Short term: -20°C
Long term: -20°C.