anti-Cat (Feline) Tumor Necrosis Factor antibody for Immunofluorescence

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Epitope
AA 115-130, N-Term
91
67
52
49
25
25
25
19
17
15
10
10
8
7
7
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
1510
551
482
226
218
199
198
62
55
42
31
18
13
13
9
7
6
4
4
4
3
3
2
1
1
1
Host
Mouse
1159
573
173
48
11
10
8
7
5
2
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to CF®647
190
102
82
63
61
39
33
31
28
26
26
26
26
25
25
25
24
20
13
11
11
11
11
11
11
11
11
11
11
11
11
11
11
10
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
913
894
792
492
401
318
222
176
133
122
95
67
48
42
26
26
25
17
17
12
10
9
9
7
6
6
6
5
5
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN6171836
$ 209.23
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN6171840 FACS IF IHC CF®660R Mouse IgM AA 115-130, N-Term Log in to see TNF706 P-T2 0
1 ABIN6171842 FACS IF IHC CF®568 Mouse IgM AA 115-130, N-Term Log in to see TNF706 P-T2 0
1 ABIN6171874 FACS IF IHC CF®647 Mouse IgM, kappa N-Term Log in to see TNF706 0
1 ABIN6171876 FACS IF IHC CF®555 Mouse IgM, kappa N-Term Log in to see TNF706 0
1 ABIN6171880 FACS IF IHC CF®568 Mouse IgM, kappa N-Term Log in to see TNF706 0
1 ABIN6171830 FACS IF IHC CF405M Mouse IgM AA 115-130, N-Term Log in to see TNF706 P-T2 0
1 ABIN6171848 FACS IF IHC CF®680R Mouse IgM AA 115-130, N-Term Log in to see TNF706 P-T2 0
1 ABIN6171866 FACS IF IHC CF®405S Mouse IgM, kappa N-Term Log in to see TNF706 0
1 ABIN6171868 FACS IF IHC CF405M Mouse IgM, kappa N-Term Log in to see TNF706 0
1 ABIN6171834 FACS IF IHC CF®543 Mouse IgM AA 115-130, N-Term Log in to see TNF706 P-T2 0
1 ABIN6171882 FACS IF IHC CF®770 Mouse IgM, kappa N-Term Log in to see TNF706 0
1 ABIN6171852 FACS IF IHC CF®594 Mouse IgM AA 115-130, N-Term Log in to see TNF706 P-T2 0
1 ABIN6171580 FACS IF IHC CF®680 Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0
1 ABIN6171566 FACS IF IHC CF®640R Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0
1 ABIN6171562 FACS IF IHC CF®405S Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0
1 ABIN6171582 FACS IF IHC CF®680R Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0
1 ABIN6171574 FACS IF IHC CF®660R Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0
1 ABIN6171584 FACS IF IHC CF®488A Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0
1 ABIN6171568 FACS IF IHC CF®543 Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0
1 ABIN6171718 FACS IF IHC CF®640R Mouse IgM, kappa AA 115-130 Log in to see P-T2 0

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 115-130, N-Term
(91), (67), (52), (49), (25), (25), (25), (19), (17), (15), (10), (10), (8), (7), (7), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(1510), (551), (482), (226), (218), (199), (198), (62), (55), (42), (31), (18), (13), (13), (9), (7), (6), (4), (4), (4), (3), (3), (2), (1), (1), (1)
Host Mouse
(1159), (573), (173), (48), (11), (10), (8), (7), (5), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is conjugated to CF®647
(190), (102), (82), (63), (61), (39), (33), (31), (28), (26), (26), (26), (26), (25), (25), (25), (24), (20), (13), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (10), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
(913), (894), (792), (492), (401), (318), (222), (176), (133), (122), (95), (67), (48), (42), (26), (26), (25), (17), (17), (12), (10), (9), (9), (7), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Purpose Mouse Monoclonal anti-TNF-alpha (TNF706 + P/T2), CF647 Conjugate
Characteristics TNF alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha causes cytolysis of certain transformed cells. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen Recombinant N-terminal fragment of human TNF-? (TNF706), Peptide corresponding to aa115-130 of human TNF-? (P/T2)
Clone TNF706 P-T2
Isotype IgM
Plasmids, Primers & others

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Molecular Weight 17 kDa
Gene ID 7124, 241570
UniProt P01375
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Immunohistology formalin-paraffin 2-4 μg/mL
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Immunofluorescence 1-2 μg/mL
  • Optimal dilution for a specific application should be determined by user
Comment

HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil or Histiocytoma

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light