anti-Human Tumor Necrosis Factor antibody for Neutralization

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Reactivity
Human
1273
459
356
144
136
119
118
62
54
41
29
17
14
12
9
7
6
4
4
4
3
2
1
1
1
1
Host
Rabbit
948
528
177
50
10
9
8
7
5
3
2
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
172
96
73
51
49
33
31
28
28
26
26
26
26
25
25
25
24
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Neutralization (Neut), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
915
864
621
324
288
230
224
190
142
107
97
67
29
29
26
22
20
18
14
11
9
7
6
6
6
6
5
5
3
3
3
3
2
2
2
2
2
2
1
1
1
1
Options
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Catalog No. ABIN1043796
$ 139.00
Plus shipping costs $45.00 and $23.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN222906 IHC Neut WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2682656 Neut Rabbit IgG AA 77-233 Log in to see R101 0
1 ABIN465605 ELISA IHC (p) Neut Mouse IgG1 Log in to see 0
1 ABIN465674 IHC (p) Neut ELISA WB Goat Log in to see Polyclonal 0
1 ABIN465030 ELISA IHC (p) Neut WB Rabbit Log in to see Polyclonal 0
1 ABIN1104720 IHC (p) Neut WB Rabbit Log in to see Polyclonal 0
1 ABIN1177286 Neut ELISA Mouse IgG2a, kappa Log in to see MABTNF-A5 3
1 ABIN1176946 Neut ELISA Mouse IgG1 Log in to see MAb1 2
1 ABIN107946 IHC Neut ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1449154 Neut Mouse IgG1 Log in to see #1 0
1 ABIN5526231 ELISA Neut HRP Mammalian Cells IgG4 kappa Log in to see CDP 571 0
1 ABIN1959982 BR ELISA Func Neut WB Mouse IgG1 Log in to see MAb1 0
1 ABIN1967143 BR ELISA Func Neut WB Mouse IgG1 Log in to see MAb1 0
1 ABIN1959983 BR ELISA FACS Func ICC IHC (fro) Neut Mouse IgG1 Log in to see MAb11 0
1 ABIN3026402 FACS IF InhA Neut Mouse IgG1, kappa Log in to see J1D9 0
1 ABIN3026400 FACS IF InhA Neut Mouse IgG1, kappa Log in to see J2D10 0
1 ABIN465677 ELISA IHC (p) Neut WB Biotin Rabbit Log in to see Polyclonal 0
1 ABIN2744953 IHC (p) Neut ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5682314 BP ELISA IF IHC Neut Human IgG1 kappa Log in to see D2E7 (Adalimumab) 0
1 ABIN5682315 BP ELISA IF IHC Neut Rabbit IgG kappa Log in to see D2E7 (Adalimumab) 0

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human
(1273), (459), (356), (144), (136), (119), (118), (62), (54), (41), (29), (17), (14), (12), (9), (7), (6), (4), (4), (4), (3), (2), (1), (1), (1), (1)
Host Rabbit
(948), (528), (177), (50), (10), (9), (8), (7), (5), (3), (2)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(172), (96), (73), (51), (49), (33), (31), (28), (28), (26), (26), (26), (26), (25), (25), (25), (24), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Neutralization (Neut), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(915), (864), (621), (324), (288), (230), (224), (190), (142), (107), (97), (67), (29), (29), (26), (22), (20), (18), (14), (11), (9), (7), (6), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN1043796) Images
Specificity This antibody is primarily directed against mature 17,000 MW human TNFalpha and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFalpha in the same formats using similar dilutions. The antibody does not recognize human TNGß (lymphotoxin). This IgG fraction antibody will recognize the cell-bound precursor of TNFalpha as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFalpha activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Characteristics Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-alpha) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).
Sterility Sterile filtered
Immunogen The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFalpha produced in E.coli.
Immunogen Type: RecombinantProtein
Isotype IgG

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN1043796) Images back to top
Antigen
Alternative Name TNF alpha (TNF Antibody Abstract)
Background Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-α) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).
Synonyms: APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody
Gene ID 7124
UniProt P01375
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor antibody (ABIN1043796) Images back to top
Application Notes This IgG fraction antibody of anti-Human TNFα has been tested for use in neutralizations, ELISA, immunohistochemistry and immunoblotting. It recognizes the 17,000 MW TNFα. Reactivity in other immunoassays is unknown.
Comment

Gene Name: TNFA

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor antibody (ABIN1043796) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

References for anti-Tumor Necrosis Factor antibody (ABIN1043796)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Kant, Swat, Zhang, Zhang, Neel, Flavell, Davis: "TNF-stimulated MAP kinase activation mediated by a Rho family GTPase signaling pathway." in: Genes & development, Vol. 25, Issue 19, pp. 2069-78, 2011

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN1043796) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN1043796) Immunohistochemistry using polyclonal TNFa antibody showing staining of formalin/PFA-...
 image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN1043796) anti-Tumor Necrosis Factor (TNF) antibody (Image 2)
 image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN1043796) anti-Tumor Necrosis Factor (TNF) antibody (Image 3)
Immunofluorescence (IF) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN1043796) Fluorescent immunohistochemistry showing staining of human colon by anti-TNF alpha (...
Western Blotting (WB) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN1043796) Western blot of Anti-Human TNF-a (RABBIT) Antibody. Lane 1: 250ng human recombinant T...
Western Blotting (WB) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN1043796) Western Blot of Rabbit anti-TNF Alpha antibody. Marker: Opal Pre-stained ladder . Lan...
Western Blotting (WB) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN1043796) Western blot using Anti-Human TNF-a (RABBIT) Antibody. Membrane blocked in 1% BSA-TB...
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN1043796) Immunohistochemistry using polyclonal TNFa antibody showing staining of formalin/PFA...