anti-Human Tumor Necrosis Factor antibody for Neutralization

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Reactivity
Human
1502
553
484
225
217
198
197
62
55
42
31
18
13
13
9
7
6
4
4
4
3
3
2
1
1
1
Host
Rabbit
1151
572
178
48
11
10
8
7
5
2
2
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
192
103
83
63
61
39
33
31
28
26
26
26
26
25
25
25
24
20
13
11
11
11
11
11
11
11
11
11
11
11
11
11
10
10
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Neutralization (Neut), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
912
891
794
488
398
315
220
176
131
122
93
67
48
42
26
26
24
17
17
12
10
9
9
7
6
6
6
5
5
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN5596796
$ 389.00
Plus shipping costs $45.00 and $23.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN222906 IHC Neut WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2682656 Neut Rabbit IgG AA 77-233 Log in to see R101 0
1 ABIN465605 ELISA Neut Mouse IgG1 Log in to see 0
1 ABIN465674 ELISA Neut WB Goat Log in to see Polyclonal 0
1 ABIN465030 ELISA Neut WB Rabbit Log in to see Polyclonal 0
1 ABIN1104720 IHC IHC (p) Neut WB Rabbit Log in to see Polyclonal 0
1 ABIN199738 FACS Neut ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1177286 Neut ELISA Mouse IgG2a, kappa Log in to see MABTNF-A5 3
1 ABIN1176946 Neut ELISA Mouse IgG1 Log in to see MAb1 2
1 ABIN1959983 BR ELISA FACS Func ICC IHC IHC (fro) Neut Mouse IgG1 Log in to see MAb11 0
1 ABIN1967143 BR ELISA Func Neut WB Mouse IgG1 Log in to see MAb1 0
1 ABIN1959982 BR ELISA Func Neut WB Mouse IgG1 Log in to see MAb1 0
1 ABIN1449154 Neut Mouse IgG1 Log in to see #1 0
1 ABIN5526231 ELISA Neut HRP Mammalian Cells IgG4 kappa Log in to see CDP 571 0
1 ABIN4360964 BP CyTOF ELISA FACS ICC IF IHC (fro) IP Neut WB Mouse IgM kappa Log in to see MAb1 0
1 ABIN465677 ELISA Neut WB Biotin Rabbit Log in to see Polyclonal 0
1 ABIN2744953 ELISA IHC IHC (p) Neut WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN603912 Neut ELISA Mouse IgG2a Log in to see 0
1 ABIN199734 ICC Neut ELISA WB Mouse IgG1 Log in to see 0
1 ABIN4248396 BP FACS Neut WB PerCP Mouse IgG1 kappa Log in to see 6N1E7 0

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human
(1502), (553), (484), (225), (217), (198), (197), (62), (55), (42), (31), (18), (13), (13), (9), (7), (6), (4), (4), (4), (3), (3), (2), (1), (1), (1)
Host Rabbit
(1151), (572), (178), (48), (11), (10), (8), (7), (5), (2), (2)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(192), (103), (83), (63), (61), (39), (33), (31), (28), (26), (26), (26), (26), (25), (25), (25), (24), (20), (13), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (10), (10), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Neutralization (Neut), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(912), (891), (794), (488), (398), (315), (220), (176), (131), (122), (93), (67), (48), (42), (26), (26), (24), (17), (17), (12), (10), (9), (9), (7), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN5596796) Images
Specificity This antibody is primarily directed against mature 17,000 MW human TNFalpha and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFalpha in the same formats using similar dilutions. The antibody does not recognize human TNGß (lymphotoxin). This IgG fraction antibody will recognize the cell-bound precursor of TNFalpha as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFalpha activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Characteristics Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-alpha) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).
Sterility Sterile filtered
Immunogen The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFalpha produced in E.coli.
Immunogen Type: RecombinantProtein
Isotype IgG
Plasmids, Primers & others

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN5596796) Images back to top
Antigen
Alternative Name TNF alpha (TNF Antibody Abstract)
Background Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-α) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).
Synonyms: APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody
Gene ID 7124
UniProt P01375
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor antibody (ABIN5596796) Images back to top
Application Notes This IgG fraction antibody of anti-Human TNFα has been tested for use in neutralizations, ELISA, immunohistochemistry and immunoblotting. It recognizes the 17,000 MW TNFα. Reactivity in other immunoassays is unknown.
Comment

Gene Name: TNFA

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor antibody (ABIN5596796) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

References for anti-Tumor Necrosis Factor antibody (ABIN5596796)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Kant, Swat, Zhang, Zhang, Neel, Flavell, Davis: "TNF-stimulated MAP kinase activation mediated by a Rho family GTPase signaling pathway." in: Genes & development, Vol. 25, Issue 19, pp. 2069-78, 2011

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN5596796) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN5596796) Immunohistochemistry using polyclonal TNFa antibody showing staining of formalin/PFA-...
 image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN5596796) anti-Tumor Necrosis Factor (TNF) antibody (Image 2)
 image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN5596796) anti-Tumor Necrosis Factor (TNF) antibody (Image 3)
Immunofluorescence (IF) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN5596796) Fluorescent immunohistochemistry showing staining of human colon by anti-TNF alpha (...
Western Blotting (WB) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN5596796) Western blot of Anti-Human TNF-a (RABBIT) Antibody. Lane 1: 250ng human recombinant T...
Western Blotting (WB) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN5596796) Western Blot of Rabbit anti-TNF Alpha antibody. Marker: Opal Pre-stained ladder . Lan...
Western Blotting (WB) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN5596796) Western blot using Anti-Human TNF-a (RABBIT) Antibody. Membrane blocked in 1% BSA-TB...
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN5596796) Immunohistochemistry using polyclonal TNFa antibody showing staining of formalin/PFA...