anti-Mouse (Murine) Tumor Necrosis Factor antibody for Enzyme Immunoassay

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Reactivity
Mouse (Murine)
1258
460
357
144
136
119
118
62
54
41
29
17
14
12
9
7
6
4
4
4
3
2
1
1
1
1
Host
Rat
937
522
183
46
10
9
7
7
5
3
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
168
100
74
50
49
32
30
28
27
25
25
25
25
25
24
24
24
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
893
853
625
323
286
229
222
190
137
118
97
68
29
29
26
22
20
18
14
9
9
7
6
6
6
6
5
5
3
3
3
3
2
2
2
2
2
2
1
1
1
1
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Catalog No. ABIN118714
$ 720.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN119359 EIA FACS IHC (fro) Rat IgG1 Log in to see MP6-XT22 5
1 ABIN118027 EIA Func IHC (fro) IHC (p) IP RIA WB Rabbit Log in to see Polyclonal 2
1 ABIN119358 EIA Func FACS IHC (fro) IHC (p) Rat IgG1 Log in to see MP6-XT22 7
1 ABIN1109383 Func EIA IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see 52B83 4
1 ABIN118025 EIA Func IHC (fro) IP RIA WB Rabbit Log in to see Polyclonal 2
1 ABIN115913 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN115914 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN1109399 Func EIA IHC (fro) IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN115911 EIA WB Biotin Rabbit Log in to see Polyclonal
1 ABIN115912 EIA WB Biotin Rabbit Log in to see Polyclonal
1 ABIN5566621 EIA RIA Biotin Rabbit AA 80-235 Log in to see Polyclonal
1 ABIN5566622 EIA RIA Biotin Rabbit AA 80-235 Log in to see Polyclonal
1 ABIN5566617 EIA RIA Rabbit AA 80-235 Log in to see Polyclonal
1 ABIN5566618 EIA RIA Rabbit AA 80-235 Log in to see Polyclonal

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Mouse (Murine)
(1258), (460), (357), (144), (136), (119), (118), (62), (54), (41), (29), (17), (14), (12), (9), (7), (6), (4), (4), (4), (3), (2), (1), (1), (1), (1)
Host Rat
(937), (522), (183), (46), (10), (9), (7), (7), (5), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(168), (100), (74), (50), (49), (32), (30), (28), (27), (25), (25), (25), (25), (25), (24), (24), (24), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(893), (853), (625), (323), (286), (229), (222), (190), (137), (118), (97), (68), (29), (29), (26), (22), (20), (18), (14), (9), (9), (7), (6), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN118714) Images
Purification Affinity chromatography on Protein G
Immunogen Recombinant murine TNF-alpha expressed in E. coli.
Clone MP6-XT3
Isotype IgG1

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN118714) Images back to top
Antigen
Alternative Name Tumor Necrosis Factor (TNF-Alpha) (TNF Antibody Abstract)
Background This 17 kDa cytokine is produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26 kDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumour cell lines, and has an important immunoregulatory role.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
Gene ID 10090
UniProt P06804
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor antibody (ABIN118714) Images back to top
Application Notes Western Blot. ELISA: 5 μg/mL. Flow Cytometry: Membrane permeabilisation is required for this application.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor antibody (ABIN118714) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Tumor Necrosis Factor antibody (ABIN118714)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Background publications

Bennouna, Bliss, Curiel, Denkers: "Cross-talk in the innate immune system: neutrophils instruct recruitment and activation of dendritic cells during microbial infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 11, pp. 6052-8, 2003

Bauman, Huffnagle, Murphy: "Effects of tumor necrosis factor alpha on dendritic cell accumulation in lymph nodes draining the immunization site and the impact on the anticryptococcal cell-mediated immune response." in: Infection and immunity, Vol. 71, Issue 1, pp. 68-74, 2002

Tsao, Kanakamma, Luh, Chou, Lei: "Inhibition of Escherichia coli-induced meningitis by carboxyfullerence." in: Antimicrobial agents and chemotherapy, Vol. 43, Issue 9, pp. 2273-7, 1999

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992