anti-Mouse (Murine) Tumor Necrosis Factor antibody for Flow Cytometry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Reactivity
Mouse (Murine)
1274
458
356
144
136
119
118
62
54
41
29
17
14
12
9
7
6
4
4
4
3
2
1
1
1
1
Host
Rat
948
529
176
50
10
9
8
7
5
3
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
172
96
73
51
49
33
31
28
28
26
26
26
26
25
25
25
24
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS)
916
865
620
324
289
230
224
190
143
107
97
67
29
29
26
22
20
18
14
11
9
7
6
6
6
6
5
5
3
3
3
3
2
2
2
2
2
2
1
1
1
1
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Catalog No. ABIN2666023
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.952866 ABIN2657589 FACS Alexa Fluor 488 Rat IgG1 kappa Log in to see MP6-XT22 11
13.952866 ABIN2662037 FACS FITC Rat IgG1 kappa Log in to see MP6-XT22 11
13.952866 ABIN2658837 FACS APC Rat IgG1 kappa Log in to see MP6-XT22 11
13.952866 ABIN2658025 FACS Alexa Fluor 647 Rat IgG1 kappa Log in to see MP6-XT22 11
13.952866 ABIN2659612 FACS PE-Cy7 Rat IgG1 kappa Log in to see MP6-XT22 11
13.952866 ABIN2662375 FACS Pacific Blue Rat IgG1 kappa Log in to see MP6-XT22 11
13.952866 ABIN2663942 FACS PE Rat IgG1 kappa Log in to see MP6-XT22 11
13.952866 ABIN4898907 FACS Fluorescein Rat IgG1 Log in to see MP6-XT22 1
13.952866 ABIN2657288 FACS Alexa Fluor 700 Rat IgG1 kappa Log in to see MP6-XT22 11
13.952866 ABIN2665434 FACS Rat IgG1 kappa Log in to see MP6-XT22 11
13.952866 ABIN4898908 FACS PE Rat IgG1 Log in to see MP6-XT22 0
13.952866 ABIN4248387 ELISA FACS WB Rat IgG1 kappa Log in to see MP6-XT22 0
1 ABIN4900489 CyTOF ELISA (Capture) FACS ICC Neut WB Goat IgG AA 80-235 Log in to see Polyclonal 53
1 ABIN2661303 FACS Biotin Rat IgG1 kappa Log in to see MP6-XT22 11
1 ABIN2665893 FACS IHC Hamster IgG Log in to see TN3-19-12 9
1 ABIN2661302 FACS Biotin Rat IgG1 kappa Log in to see MP6-XT22 14
1 ABIN2665424 FACS ICFC Hamster IgG Log in to see TN3-19-12 9
1 ABIN4360905 FACS IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see 52B83 3
1 ABIN2663943 FACS PE Rat IgG1 kappa Log in to see MP6-XT22 12
1 ABIN2662038 FACS FITC Rat IgG1 kappa Log in to see MP6-XT22 12

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Mouse (Murine)
(1274), (458), (356), (144), (136), (119), (118), (62), (54), (41), (29), (17), (14), (12), (9), (7), (6), (4), (4), (4), (3), (2), (1), (1), (1), (1)
Host Rat
(948), (529), (176), (50), (10), (9), (8), (7), (5), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(172), (96), (73), (51), (49), (33), (31), (28), (28), (26), (26), (26), (26), (25), (25), (25), (24), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS)
(916), (865), (620), (324), (289), (230), (224), (190), (143), (107), (97), (67), (29), (29), (26), (22), (20), (18), (14), (11), (9), (7), (6), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 11 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN2666023) Images
Brand Ultra-LEAF™
Purification The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level

Endotoxin level is <0.01 EU/μg of the protein (<0.001 ng/μg of the protein) as determined by the LAL test.

Clone MP6-XT22
Isotype IgG1 kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN2666023) Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is a 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD. TNF-α forms multimeric complexes, stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor antibody (ABIN2666023) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor antibody (ABIN2666023) Images back to top
Concentration 1.0 mg/mL
Preservative Azide free
Handling Advice This Ultra-LEAF™ solution contains no preservative, handle under aseptic conditions
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-Tumor Necrosis Factor antibody (ABIN2666023)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Background publications

Via, Shustov, Rus, Lang, Nguyen, Finkelman: "In vivo neutralization of TNF-alpha promotes humoral autoimmunity by preventing the induction of CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 12, pp. 6821-6, 2001

Infante-Duarte, Horton, Byrne, Kamradt: "Microbial lipopeptides induce the production of IL-17 in Th cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 11, pp. 6107-15, 2000

Jacobs, Brown, Allie, Gulert, Ryffel: "Increased resistance to mycobacterial infection in the absence of interleukin-10." in: Immunology, Vol. 100, Issue 4, pp. 494-501, 2000

Williams, Marinova-Mutafchieva, Feldmann, Maini: "Evaluation of TNF-alpha and IL-1 blockade in collagen-induced arthritis and comparison with combined anti-TNF-alpha/anti-CD4 therapy." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 12, pp. 7240-5, 2000

Marinova-Mutafchieva, Williams, Mason, Mauri, Feldmann, Maini: "Dynamics of proinflammatory cytokine expression in the joints of mice with collagen-induced arthritis (CIA)." in: Clinical and experimental immunology, Vol. 107, Issue 3, pp. 507-12, 1997

Mo, Sarawar, Doherty: "Induction of cytokines in mice with parainfluenza pneumonia." in: Journal of virology, Vol. 69, Issue 2, pp. 1288-91, 1995

Sarawar, Sangster, Coffman, Doherty: "Administration of anti-IFN-gamma antibody to beta 2-microglobulin-deficient mice delays influenza virus clearance but does not switch the response to a T helper cell 2 phenotype." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 3, pp. 1246-53, 1994

Tracey, Cerami: "Tumor necrosis factor: an updated review of its biology." in: Critical care medicine, Vol. 21, Issue 10 Suppl, pp. S415-22, 1993

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Beutler, Cerami: "The biology of cachectin/TNF--a primary mediator of the host response." in: Annual review of immunology, Vol. 7, pp. 625-55, 1989

Beutler, Cerami: "Tumor necrosis, cachexia, shock, and inflammation: a common mediator." in: Annual review of biochemistry, Vol. 57, pp. 505-18, 1988

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN2666023) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN2666023) anti-Tumor Necrosis Factor (TNF) antibody
Immunofluorescence (IF) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN2666023) anti-Tumor Necrosis Factor (TNF) antibody (Image 2)