anti-Rabbit Tumor Necrosis Factor antibody for Immunofluorescence

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LOC103694380
  • tnfa
Epitope
N-Term
97
69
52
49
26
22
19
15
14
10
10
8
8
7
6
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
1380
529
458
228
222
202
201
59
56
44
32
18
15
9
7
6
6
5
4
4
4
4
3
2
2
1
1
1
1
Host
Mouse
1070
544
138
52
11
10
8
7
5
2
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to APC
185
95
71
55
48
26
23
22
21
20
19
19
19
19
19
19
18
17
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
831
807
755
468
404
269
206
170
134
120
100
67
48
42
26
26
25
17
15
13
9
9
9
7
6
6
6
5
5
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN6171892
$ 392.62
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN6171899 FACS IF IHC PerCP Mouse IgM, kappa N-Term Log in to see TNF706 0
7 ABIN6171900 FACS IF IHC PE Mouse IgM, kappa N-Term Log in to see TNF706 0
7 ABIN6171903 FACS IF IHC Mouse IgM, kappa N-Term Log in to see TNF706 0
7 ABIN3023989 FACS IF IHC (p) Mouse IgM, kappa N-Term Log in to see TNF706 0
4 ABIN6171595 FACS IF IHC PerCP Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0
4 ABIN6171596 FACS IF IHC PE Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0
4 ABIN6171740 FACS IF IHC APC Mouse IgM, kappa AA 115-130 Log in to see P-T2 0
4 ABIN6171747 FACS IF IHC PerCP Mouse IgM, kappa AA 115-130 Log in to see P-T2 0
4 ABIN6171748 FACS IF IHC PE Mouse IgM, kappa AA 115-130 Log in to see P-T2 0
4 ABIN6171588 FACS IF IHC APC Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0
4 ABIN6171599 FACS IF IHC Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0
4 ABIN6171751 FACS IF IHC Mouse IgM, kappa AA 115-130 Log in to see P-T2 0
4 ABIN3023960 FACS IF IHC (p) Mouse IgM AA 115-130, N-Term Log in to see TNF706-P-T2 0
4 ABIN3023987 FACS IF IHC (p) Mouse IgM, kappa AA 115-130 Log in to see P-T2 0
4 ABIN3023991 FACS IF IHC (p) Mouse IgG1, kappa AA 115-130 Log in to see SPM543 0
1 ABIN6171584 FACS IF IHC CF®488A Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0
1 ABIN6171570 FACS IF IHC CF®647 Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0
1 ABIN6171562 FACS IF IHC CF®405S Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0
1 ABIN6171566 FACS IF IHC CF®640R Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0
1 ABIN6171574 FACS IF IHC CF®660R Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope N-Term
(97), (69), (52), (49), (26), (22), (19), (15), (14), (10), (10), (8), (8), (7), (6), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(1380), (529), (458), (228), (222), (202), (201), (59), (56), (44), (32), (18), (15), (9), (7), (6), (6), (5), (4), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(1070), (544), (138), (52), (11), (10), (8), (7), (5), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is conjugated to APC
(185), (95), (71), (55), (48), (26), (23), (22), (21), (20), (19), (19), (19), (19), (19), (19), (18), (17), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
(831), (807), (755), (468), (404), (269), (206), (170), (134), (120), (100), (67), (48), (42), (26), (26), (25), (17), (15), (13), (9), (9), (9), (7), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Purpose Mouse Monoclonal anti-TNF-alpha (TNF706), APC Conjugate
Characteristics Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen Recombinant N-terminal fragment of human TNF-?
Clone TNF706
Isotype IgM, kappa
Plasmids, Primers & others

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Molecular Weight 17 kDa
Gene ID 7124, 241570
UniProt P01375
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Immunohistology formalin-paraffin 2-4 μg/mL
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Immunofluorescence 1-2 μg/mL
  • Optimal dilution for a specific application should be determined by user
Comment

HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling back to top
Supplier Images
 image for anti-Tumor Necrosis Factor (TNF) (N-Term) antibody (APC) (ABIN6171892) anti-Tumor Necrosis Factor (TNF) (N-Term) antibody (APC)
 image for anti-Tumor Necrosis Factor (TNF) (N-Term) antibody (APC) (ABIN6171892) anti-Tumor Necrosis Factor (TNF) (N-Term) antibody (APC) (Image 2)