anti-Rabbit Tumor Necrosis Factor antibody for Immunofluorescence

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Reactivity
Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
1510
552
483
225
219
199
199
61
55
43
31
17
14
13
9
7
6
4
4
4
4
3
2
2
1
1
1
1
Host
Mouse
1157
574
173
49
11
10
8
7
5
2
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to Alexa Fluor 488
190
102
83
63
61
39
32
31
28
26
26
26
26
25
25
25
24
20
13
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
906
894
792
492
402
318
221
176
133
120
95
67
48
42
26
26
25
17
17
12
10
9
9
7
6
6
6
5
5
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN4360976
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN6171836 FACS IF IHC CF®647 Mouse IgM AA 115-130, N-Term Log in to see TNF706 P-T2 0
1 ABIN6171840 FACS IF IHC CF®660R Mouse IgM AA 115-130, N-Term Log in to see TNF706 P-T2 0
1 ABIN6171842 FACS IF IHC CF®568 Mouse IgM AA 115-130, N-Term Log in to see TNF706 P-T2 0
1 ABIN6171874 FACS IF IHC CF®647 Mouse IgM, kappa N-Term Log in to see TNF706 0
1 ABIN6171876 FACS IF IHC CF®555 Mouse IgM, kappa N-Term Log in to see TNF706 0
1 ABIN6171880 FACS IF IHC CF®568 Mouse IgM, kappa N-Term Log in to see TNF706 0
1 ABIN6171830 FACS IF IHC CF405M Mouse IgM AA 115-130, N-Term Log in to see TNF706 P-T2 0
1 ABIN6171848 FACS IF IHC CF®680R Mouse IgM AA 115-130, N-Term Log in to see TNF706 P-T2 0
1 ABIN6171866 FACS IF IHC CF®405S Mouse IgM, kappa N-Term Log in to see TNF706 0
1 ABIN6171868 FACS IF IHC CF405M Mouse IgM, kappa N-Term Log in to see TNF706 0
1 ABIN6171834 FACS IF IHC CF®543 Mouse IgM AA 115-130, N-Term Log in to see TNF706 P-T2 0
1 ABIN6171882 FACS IF IHC CF®770 Mouse IgM, kappa N-Term Log in to see TNF706 0
1 ABIN6171852 FACS IF IHC CF®594 Mouse IgM AA 115-130, N-Term Log in to see TNF706 P-T2 0
1 ABIN6171580 FACS IF IHC CF®680 Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0
1 ABIN6171566 FACS IF IHC CF®640R Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0
1 ABIN6171562 FACS IF IHC CF®405S Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0
1 ABIN6171582 FACS IF IHC CF®680R Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0
1 ABIN6171574 FACS IF IHC CF®660R Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0
1 ABIN6171584 FACS IF IHC CF®488A Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0
1 ABIN6171568 FACS IF IHC CF®543 Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8 0

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(1510), (552), (483), (225), (219), (199), (199), (61), (55), (43), (31), (17), (14), (13), (9), (7), (6), (4), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(1157), (574), (173), (49), (11), (10), (8), (7), (5), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is conjugated to Alexa Fluor 488
(190), (102), (83), (63), (61), (39), (32), (31), (28), (26), (26), (26), (26), (25), (25), (25), (24), (20), (13), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(906), (894), (792), (492), (402), (318), (221), (176), (133), (120), (95), (67), (48), (42), (26), (26), (25), (17), (17), (12), (10), (9), (9), (7), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Specificity Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Purification Protein A purified
Immunogen A hexadecapeptide corresponding to aa115-130 of human TNF-alpha, conjugated to thyroglobulin
Clone SPM543
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background Gene Symbol: TNF
Molecular Weight Theoretical MW: 17 kDa
Gene ID 7124
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-FrozenImmunohistochemistry (Staining of paraffin tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Format Liquid
Buffer 50 mM Sodium Borate
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C in the dark.

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor (TNF) antibody (Alexa Fluor 488) (ABIN4360976) Flow Cytometry: TNF-alpha Antibody (SPM543) - An intracellular stain was performed ...