General
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Antigen
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Tumor Necrosis Factor (TNF) Antibodies
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Epitope
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AA 1-234
Alternatives
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Reactivity
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Human, Rat (Rattus)
Alternatives
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Host
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Mouse
Alternatives
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Clonality (Clone)
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Conjugate
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This Tumor Necrosis Factor antibody is un-conjugated
Alternatives
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Application
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Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Alternatives
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Supplier
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Log in to see
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Specificity
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This antibody reacts to TNF.
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Purification
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Affinity chromatography on Protein A
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Immunogen
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TNF antibody was raised against tNF (AAH28148, 1 a.a. ~ 234 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
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Clone
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M1-C4
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Isotype
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IgG1
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Plasmids, Primers & others
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Antigen
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Alternative Name
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Tumor Necrosis Factor (TNF-Alpha) (TNF Antibody Abstract)
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Background
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Tumor Necrosis Factor (TNF) is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. TNF is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
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Gene ID
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7124
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NCBI Accession
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NP_000585
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UniProt
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P01375
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Pathways
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NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus
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Application Notes
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Optimal working dilution should be determined by the investigator.
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Restrictions
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For Research Use only
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Concentration
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0,5 mg/mL
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Buffer
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PBS, pH 7.2
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Handling Advice
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Avoid repeated freezing and thawing.
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Storage
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4 °C/-20 °C
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Storage Comment
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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Supplier Images
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