anti-Rat (Rattus) Tumor Necrosis Factor antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Epitope
AA 180-230
90
68
52
49
25
25
25
19
17
14
10
10
8
7
7
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1502
552
483
225
217
198
197
62
55
42
31
18
13
13
9
7
6
4
4
4
3
3
2
1
1
1
Host
Rabbit
1151
572
178
48
11
10
8
7
5
2
2
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
192
103
83
63
61
39
33
31
28
26
26
26
26
25
25
25
24
20
13
11
11
11
11
11
11
11
11
11
11
11
11
11
10
10
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
913
891
794
489
398
314
220
176
132
122
93
67
48
42
26
25
24
17
17
12
10
9
9
7
6
6
6
5
5
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Options
Supplier
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Catalog No. ABIN677318
$ 318.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2807091 IF (p) Alexa Fluor 594 Rabbit IgG AA 180-230 Log in to see Polyclonal 0
1 ABIN918032 IF (p) Alexa Fluor 350 Rabbit IgG AA 180-230 Log in to see Polyclonal 0
1 ABIN918034 IF (p) Alexa Fluor 488 Rabbit IgG AA 180-230 Log in to see Polyclonal 0
1 ABIN677321 IF (p) Cy3 Rabbit IgG AA 180-230 Log in to see Polyclonal 0
1 ABIN677325 IF (p) FITC Rabbit IgG AA 180-230 Log in to see Polyclonal 0
1 ABIN677324 IF (p) Cy7 Rabbit IgG AA 180-230 Log in to see Polyclonal 0
1 ABIN677322 IF (p) Cy5 Rabbit IgG AA 180-230 Log in to see Polyclonal 0
1 ABIN677323 IF (p) Cy5.5 Rabbit IgG AA 180-230 Log in to see Polyclonal 0
1 ABIN918031 IF (p) Alexa Fluor 647 Rabbit IgG AA 180-230 Log in to see Polyclonal 0
1 ABIN918042 IF (p) Alexa Fluor 555 Rabbit IgG AA 180-230 Log in to see Polyclonal 0
1 ABIN5011738 IF (p) Alexa Fluor 680 Rabbit IgG AA 180-230 Log in to see Polyclonal 0
1 ABIN5011739 IF (p) Alexa Fluor 750 Rabbit IgG AA 180-230 Log in to see Polyclonal 0

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 180-230
(90), (68), (52), (49), (25), (25), (25), (19), (17), (14), (10), (10), (8), (7), (7), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1502), (552), (483), (225), (217), (198), (197), (62), (55), (42), (31), (18), (13), (13), (9), (7), (6), (4), (4), (4), (3), (3), (2), (1), (1), (1)
Host Rabbit
(1151), (572), (178), (48), (11), (10), (8), (7), (5), (2), (2)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(192), (103), (83), (63), (61), (39), (33), (31), (28), (26), (26), (26), (26), (25), (25), (25), (24), (20), (13), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (10), (10), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(913), (891), (794), (489), (398), (314), (220), (176), (132), (122), (93), (67), (48), (42), (26), (25), (24), (17), (17), (12), (10), (9), (9), (7), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 12 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN677318) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from mouse TNF-alpha
Isotype IgG
Plasmids, Primers & others

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN677318) Images back to top
Antigen
Alternative Name Tnf alpha (TNF Antibody Abstract)
Background

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Subcellular location: Extracellular

Synonyms: DIF, Tnfa, TNF-a, TNFSF2, Tnfsf1a, TNFalpha, TNF-alpha, Tumor necrosis factor, Cachectin, Tumor necrosis factor ligand superfamily member 2, Tnf

Gene ID 21926
UniProt P06804
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor antibody (ABIN677318) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor antibody (ABIN677318) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-Tumor Necrosis Factor antibody (ABIN677318)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Dong, Zhou, Ji, Pan, Zheng: "AG1296 enhances plaque stability via inhibiting inflammatory responses and decreasing MMP-2 and MMP-9 expression in ApoE-/- mice." in: Biochemical and biophysical research communications, Vol. 489, Issue 4, pp. 426-431, 2017 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Han, Wang, Qin, Xia, Wu: "LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4." in: Journal of experimental & clinical cancer research : CR, Vol. 36, Issue 1, pp. 83, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Wang, Tang, Shen, Chen, Chen, Luo, Ge: "Hepatocyte growth factor (HGF) optimizes oral traumatic ulcer healing of mice by reducing inflammation." in: Cytokine, Vol. 99, pp. 275-280, 2017 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Shi, Yu, Liu, Liu, Zhang, Yang, Chai, Lou, Gao, Zhu: "Panax notoginseng saponins administration modulates pro- /anti-inflammatory factor expression and improves neurologic outcome following permanent MCAO in rats." in: Metabolic brain disease, Vol. 32, Issue 1, pp. 221-233, 2016 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Rat (Rattus)). Further details: Sample: brain

Ji, Wang, Wang, Chen, Li: "Protective effect of Xuebijing injection against acute lung injury induced by left ventricular ischemia/reperfusion in rabbits." in: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 51-58, 2016 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Rabbit). Further details: Sample: lung

Du, Niu, Wang, Zhao, Kong, Zhang, Nan: "TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice." in: Molecular medicine reports, Vol. 13, Issue 3, pp. 2159-66, 2016 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)). Further details: Sample: liver

Wang, Li, Wei, Xu, Chen, Song, Lu, Wang: "Targeted knockout of TNF?? by injection of lentivirus?mediated siRNA into the subacromial bursa for the treatment of subacromial bursitis in rats." in: Molecular medicine reports, Vol. 12, Issue 3, pp. 4389-95, 2015

Sun, Zeng, Cao, Song, Zhang, Liu: "Vibration Training Triggers Brown Adipocyte Relative Protein Expression in Rat White Adipose Tissue." in: BioMed research international, Vol. 2015, pp. 919401, 2015 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Xu, Zhao, Lin, Wang, Hu, Jiang: "Role of Dectin-1 in the innate immune response of rat corneal epithelial cells to Aspergillus fumigatus." in: BMC ophthalmology, Vol. 15, pp. 126, 2015 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Rat (Rattus)).

Morishita, Oku, Horie, Tonari, Kida, Okubo, Sugiyama, Takai, Hara, Ikeda: "Systemic simvastatin rescues retinal ganglion cells from optic nerve injury possibly through suppression of astroglial NF-?B activation." in: PLoS ONE, Vol. 9, Issue 1, pp. e84387, 2014 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Rat (Rattus)).

Wang, Hu, Wang, Gu, Gao, Cao, Zheng: "5-Aminolevulinic acid-mediated sonodynamic therapy reverses macrophage and dendritic cell passivity in murine melanoma xenografts." in: Ultrasound in medicine & biology, Vol. 40, Issue 9, pp. 2125-33, 2014 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Wang, Zhang, Gu, Ding, Fan, Zhu, Shi, Hong, Kong: "Morin reduces hepatic inflammation-associated lipid accumulation in high fructose-fed rats via inhibiting sphingosine kinase 1/sphingosine 1-phosphate signaling pathway." in: Biochemical pharmacology, Vol. 86, Issue 12, pp. 1791-804, 2013 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)). Further details: Sample: liver

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN677318) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Antigen: 0.2 µg/100 µL Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:...
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha P...
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Formalin-fixed and paraffin embedded rat hippocampus labeled with Rabbit Anti-TNF alp...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha P...
Western Blotting (WB) image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Mouse liver lysates probed with TNF alpha Polyclonal Antibody, unconjugated at 1:300...
'Independent Validation' Badge
Lot Number 140113
Method validated Immunofluorescence
Positive Control Normal mouse lung tissue
Negative Control Normal mouse adipose tissue
Notes Fluorescent signal is detected in positive control, but not in negative control or isotype controls.
Primary Antibody
  • Antibody: Tumor Necrosis Factor (TNF)
  • Catalog number: ABIN677318
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 140113
Isotype Control Antibody
Secondary Antibody
  • Antibody: Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (Alexa 647)
  • Catalog number: Log in to see
  • Supplier: Log in to see
  • Lot number: Not available
Controls
  • Positive control: FFPE normal mouse lung tissue
  • Negative Control: FFPE normal mouse fat tissue
  • Isotype antibody control: tissue sections treated with Rabbit IgG Isotype at 1ug/ul
  • Secondary antibody only control: positive and negative control tissue sections treated with Goat anti-Rabbit Alexa 647 secondary antibody only. Any staining observed is due to non-specific binding of secondary antibody.
Protocol
  • Paraffin embedded positive and negative control tissue sections were deparaffinized and underwent antigen retrieval using 10 mM sodium citrate pH 6.0 in a pressure cooker for 10 min.
  • The tissue sections were incubated with 1 mg/mL sodium borohydride for 10 min at room temperature to block autofluorescent background signal. The sections were then rinsed three times in TBS for 5 min each at RT.
  • Tissue sections were blocked in 1 X TBS / 5% normal donkey serum for 60 min at RT.
  • Tissue sections were incubated with primary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum overnight at 4°C.
  • Tissue sections were rinsed three times in TBS for 5 min each at RT.
  • Cells were incubated with secondary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum for 60 min at RT in dark.
  • Tissue sections were rinsed three times in TBS for 5 min each at RT.
  • Coverslips were mounted on slides with Prolong Gold anti-fade mounting media (Invitrogen).
  • IF stained tissue sections were imaged with a Zeiss LSM 510 Meta confocal microscope.
Experimental Notes Nothing to note.
Validation Images
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 1: confocal image of positive control (mouse lung) with anti-TNF alpha antibody
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 2: confocal image of negative control (mouse adipose) with anti-TNF alpha antib...
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 3: confocal image of positive control (mouse lung) with isotype control
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 4: confocal image of negative control (mouse adipose) with isotype control
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 5: confocal image of positive control (mouse lung) with secondary antibody only