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|Antigen||Tumor Necrosis Factor (TNF) Antibodies|
|Epitope||AA 100-200, Internal Region Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Tumor Necrosis Factor antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Free Floating) (IHC (ff)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|13 references available|
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Product Details anti-Tumor Necrosis Factor AntibodyTarget Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN446837) Images
|Purification||Immunogen affinity purified|
|Immunogen||A synthetic peptide made to an internal portion of the human TNF alpha protein (between residues 100-200) [UniProt P01375]|
|Plasmids, Primers & others|
Target Details Tumor Necrosis FactorProduct Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN446837) Images back to top
|Alternative Name||TNF-alpha (TNF Antibody Abstract)|
|Background||Gene Symbol: TNF|
|Pathways||NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus|
Application DetailsProduct Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor antibody (ABIN446837) Images back to top
|Application Notes||Western Blot 1:250 - 1:500, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:200 - 1:300, Immunohistochemistry-Paraffin 1:200, Immunohistochemistry-Frozen, Immunohistochemistry Free-FloatingWestern Blot data from customer review and citation (PMID: 24223475). Use in Immunohistochemistry-free floating reported in scientific literature (PMID 26219646) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 26725948)|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Immunohistochemistry Protocol specific for TNF alpha antibody Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
1. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.Immunocytochemistry Protocol specific for TNF alpha antibody Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor antibody (ABIN446837) Images back to top
Buffer contains: 0.02 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||4 °C,-20 °C|
|Storage Comment||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
References for anti-Tumor Necrosis Factor antibody (ABIN446837)Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
|Product cited in:||
Tucker, Riggin, Connizzo, Mauck, Steinberg, Kuntz, Soslowsky, Bernstein: "Effect of overuse-induced tendinopathy on tendon healing in a rat supraspinatus repair model." in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 34, Issue 1, pp. 161-6, 2016 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Vieira Ramos, Pinheiro, Messa, Delfino, Marqueti, Salvini, Durigan: "Cryotherapy Reduces Inflammatory Response Without Altering Muscle Regeneration Process and Extracellular Matrix Remodeling of Rat Muscle." in: Scientific reports, Vol. 6, pp. 18525, 2016 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Frozen Sections)
Xu, Romero, Miller, Kadam, Mial, Plazyo, Garcia-Flores, Hassan, Xu, Tarca, Drewlo, Gomez-Lopez: "An M1-like Macrophage Polarization in Decidual Tissue during Spontaneous Preterm Labor That Is Attenuated by Rosiglitazone Treatment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 196, Issue 6, pp. 2476-91, 2016 (Sample species: Human). Further details: Flow Cytometry,Immunohistochemistry (Frozen Sections)
Szylberg, Janiczek, Popiel, Marszałek: "Expression of COX-2, IL-1β, TNF-α and IL-4 in epithelium of serrated adenoma, adenoma and hyperplastic polyp." in: Archives of medical science : AMS, Vol. 12, Issue 1, pp. 172-8, 2016 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Veruva, Lanman, Isaza, Freeman, Kurtz, Steinbeck: "Periprosthetic UHMWPE Wear Debris Induces Inflammation, Vascularization, and Innervation After Total Disc Replacement in the Lumbar Spine." in: Clinical orthopaedics and related research, 2016 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry
Acosta, Tajiri, Hoover, Kaneko, Borlongan: "Intravenous Bone Marrow Stem Cell Grafts Preferentially Migrate to Spleen and Abrogate Chronic Inflammation in Stroke." in: Stroke, Vol. 46, Issue 9, pp. 2616-27, 2015 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Free Floating)
Piao, Yuan, Wang, Sun, Liang, Ma: "α-catenin acts as a tumour suppressor in E-cadherin-negative basal-like breast cancer by inhibiting NF-κB signalling." in: Nature cell biology, Vol. 16, Issue 3, pp. 245-54, 2014 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Thomas, Sarver, Yannascoli, Tucker, Kelly, Ahima, Barbe, Soslowsky: "Effect of isolated hyperglycemia on native mechanical and biologic shoulder joint properties in a rat model." in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 32, Issue 11, pp. 1464-70, 2014 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Castoldi, di Gioia, Bombardi, Maestroni, Carletti, Steckelings, Dahlöf, Unger, Zerbini, Stella: "PREVENTION OF DIABETIC NEPHROPATHY BY COMPOUND 21, SELECTIVE AGONIST OF THE ANGIOTENSIN TYPE 2 RECEPTORS, IN ZUCKER DIABETIC FATTY RATS." in: American journal of physiology. Renal physiology, pp. ajprenal.00247.2014, 2014
Chen, Shi, Zhang, Lei, Long, Su, Pei, Huang: "Scutellarin attenuates hypertension-induced expression of brain Toll-like receptor 4/nuclear factor kappa B." in: Mediators of inflammation, Vol. 2013, pp. 432623, 2013 (Sample species: Rat (Rattus)). Further details: Western Blotting
Kwon, Jung, Hwang, Choi: "Evaluation of Bronchiolar and Alveolar Cell Injuries Induced by Short- and Long-term Exposure to Sidestream Smoke." in: Korean journal of pathology, Vol. 46, Issue 2, pp. 151-61, 2012
Mohamed, Coucha, Elshaer, Artham, Lemtalsi, El-Remessy: "Inducible overexpression of endothelial proNGF as a mouse model to study microvascular dysfunction." in: Biochimica et biophysica acta, Vol. 1864, Issue 3, pp. 746-757, 1970 (Sample species: Mouse (Murine)).
Özdemir, Altun, Arslan et al.: "Imidacloprid exposure cause the histopathological changes, activation of TNF-α, iNOS, 8-OHdG biomarkers, and alteration of caspase 3, iNOS, CYP1A, MT1 gene expression levels in common carp (Cyprinus ..." in: Toxicology reports, Vol. 5, pp. 125-133, 1970 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Fish).
ImagesProduct Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN446837) back to top