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|Antigen||Estrogen Receptor 2 (ER Beta) (ESR2) Antibodies|
|Reactivity||Human, Monkey, Mouse (Murine) Alternatives|
Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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|Specificity||Strong Erb staining in epithelial cell nuclei. Occasional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also successfully detected ERb in colon and ovarian tumors using the same antibody.|
|Purification||Protein G purified|
|Immunogen||Amino acids 1-153 of human ER-beta expressed in E. coli.|
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|Alternative Name||ER beta/nr3a2 (ESR2 Antibody Abstract)|
|Background||Gene Symbol: ESR2|
|Molecular Weight||Theoretical MW: 59 kDa|
|Pathways||Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling|
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|Application Notes||Western Blot 1-5 μg/mL, Chromatin Immunoprecipitation, Flow Cytometry 1:10 - 1:1000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation, Immunohistochemistry-Paraffin 1:10 - 1:500, Dot BlotIP usage reported in scientific literature. Strong ER beta staining in epithelial cell nuclei. Occassional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also detected ER beta in colon and ovarian tumors. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Immunohistochemistry protocol specific for Estrogen Receptor beta Antibody Immunohistochemistry Procedure
1. Cut 4-5 micron sections and mount onto Superfrost plus slides.
. Dewax in xylene and rehydrate through graded alcohols.
. Block with 3 % H2O2 in methanol for 10 minutes.
. Wash in running tap water for 5 minutes then block non-specific binding sites with normal horse serum.
. To unmask the antigen, heat-mediated retrieval is required using either a microwave or pressure cooker. For microwave-mediated retrieval immerse sections in 1 mM citrate buffer, pH 6, and microwaved at full power (600W) for 27 minutes . Alternatively, sections can be pressure cooked for 4 minutes, immersed in 1 mM citrate buffer, pH
. Allow sections to cool for 20-30 minutes and wash in running tap water for 5 minutes.
. Incubation sections with 5ug/ml primary antibody at 4C overnight. Incubation time and temperature are critical.
. Incubate with appropriate biotinylated secondary antibody for 30 minutes at room temperature, followed by streptavidin ABC kit (DAKO) according to the manufacturer's instructions.
. Prepare 3,3'-diaminobenzidine substrate and apply for 5-10 minutes.
. Counterstain with haematoxylin,dehydrate and coverslip.Using this protocol, we detect consistent, strong ERb staining in epithelial cell nuclei. Occasional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also successfully detected ERb in colon and ovarian tumours using the same antibody.
|Restrictions||For Research Use only|
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PBS ( pH 7.4)
Buffer contains: No Preservative
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||4 °C,-20 °C|
|Storage Comment||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
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|Product cited in:||
Kauffman, Robinson, Downes, Marcinkiewicz, Vourganti, Scherr, Gudas, Mongan: "Estrogen receptor-β expression and pharmacological targeting in bladder cancer." in: Oncology reports, Vol. 30, Issue 1, pp. 131-8, 2013
López-García, E Cuevas, Corona-Quintanilla, Jiménez-Estrada, Martínez-Gómez, Castelán: "Effect of multiparity on morphometry and oestrogen receptor expression of pelvic and perineal striated muscles in rabbits: is serum oestradiol relevant?" in: European journal of obstetrics, gynecology, and reproductive biology, Vol. 169, Issue 1, pp. 113-20, 2013 (Sample species: Rabbit). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Jardel, Debiais, Godet, Irani, Fromont: "Ductal carcinoma of the prostate shows a different immunophenotype from high grade acinar cancer." in: Histopathology, Vol. 63, Issue 1, pp. 57-63, 2013 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Castelán, Xelhuantzi, Hernández-Aragón, Rodríguez-Antolín, Cuevas, Martínez-Gómez: "Morphometry of paravaginal ganglia from the pelvic plexus: impact of multiparity, primiparity, and pregnancy." in: European journal of obstetrics, gynecology, and reproductive biology, Vol. 170, Issue 1, pp. 286-92, 2013 (Sample species: Rabbit). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Honma, Saji, Hirose, Horiguchi, Kuroi, Hayashi, Utsumi, Harada: "Sex steroid hormones in pairs of tumor and serum from breast cancer patients and pathobiological role of androstene-3β, 17β-diol." in: Cancer science, Vol. 102, Issue 10, pp. 1848-54, 2012 (Sample species: Human). Further details: Chromatin Immunoprecipitation
Navaratnam, Skliris, Qing, Banerji, Badiani, Tu, Bradbury, Leighl, Shepherd, Nowatzki, Demers, Murphy: "Differential role of estrogen receptor beta in early versus metastatic non-small cell lung cancer." in: Hormones & cancer, Vol. 3, Issue 3, pp. 93-100, 2012 (Sample species: Human). Further details: Chromatin Immunoprecipitation
Verma, Miki, Abe, Niikawa, Sasano: "Cytoplasmic estrogen receptor β as a potential marker in human non-small cell lung carcinoma." in: Expert opinion on therapeutic targets, Vol. 16 Suppl 1, pp. S91-102, 2012 (Sample species: Human). Further details: Chromatin Immunoprecipitation
Zuguchi, Miki, Onodera, Fujishima, Takeyama, Okamoto, Miyata, Sato, Satomi, Sasano: "Estrogen receptor α and β in esophageal squamous cell carcinoma." in: Cancer science, Vol. 103, Issue 7, pp. 1348-55, 2012 (Sample species: Human). Further details: Chromatin Immunoprecipitation
Tonetti, Gao, Escarzaga, Walters, Szafran, Coon: "PKCα and ERβ Are Associated with Triple-Negative Breast Cancers in African American and Caucasian Patients." in: International journal of breast cancer, Vol. 2012, pp. 740353, 2012 (Sample species: Human). Further details: Immunohistochemistry
Karlsson, Helenius, Fernandes, Karlsson: "Oestrogen receptor β in NSCLC - prevalence, proliferative influence, prognostic impact and smoking." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 120, Issue 6, pp. 451-8, 2012 (Sample species: Human). Further details: Chromatin Immunoprecipitation
Cheng, Li, Han, Lin, Niu, Zhou, Yuan, Huang, Li, Jiang, Zhang, Ding, Xu, Ye: "PES1 promotes breast cancer by differentially regulating ERα and ERβ." in: The Journal of clinical investigation, Vol. 122, Issue 8, pp. 2857-70, 2012 (Sample species: Human). Further details: Immunoprecipitation,Chromatin Immunoprecipitation
Sato, Suzuki, Katayose, Miura, Shiiba, Miki, Kamogawa, Yamamoto, Takayuki, Egawa, Unno, Sasano: "Aromatase in colon carcinoma." in: Anticancer research, Vol. 32, Issue 8, pp. 3069-75, 2012 (Sample species: Human). Further details: Chromatin Immunoprecipitation
Verma, Miki, Abe, Nagasaki, Niikawa, Suzuki, Kondo, Sasano: "Co-expression of estrogen receptor beta and aromatase in Japanese lung cancer patients: gender-dependent clinical outcome." in: Life sciences, Vol. 91, Issue 15-16, pp. 800-8, 2012 (Sample species: Human). Further details: Chromatin Immunoprecipitation
Sase, Suzuki, Miura, Shiiba, Sato, Nakamura, Takagi, Onodera, Miki, Watanabe, Ishida, Ohnuma, Sasaki, Sato, Karasawa, Shibata, Unno, Sasaki, Sasano: "Runt-related transcription factor 2 in human colon carcinoma: a potent prognostic factor associated with estrogen receptor." in: International journal of cancer. Journal international du cancer, Vol. 131, Issue 10, pp. 2284-93, 2012 (Sample species: Human). Further details: Chromatin Immunoprecipitation
Paruthiyil, Cvoro, Tagliaferri, Cohen, Shtivelman, Leitman: "Estrogen receptor β causes a G2 cell cycle arrest by inhibiting CDK1 activity through the regulation of cyclin B1, GADD45A, and BTG2." in: Breast cancer research and treatment, Vol. 129, Issue 3, pp. 777-84, 2011 (Sample species: Human). Further details: Chromatin Immunoprecipitation
Miki, Abe, Suzuki, Suzuki, Sasano: "Suppression of estrogen actions in human lung cancer." in: Molecular and cellular endocrinology, Vol. 340, Issue 2, pp. 168-74, 2011 (Sample species: Human). Further details: Chromatin Immunoprecipitation
Vouk, Smuc, Guggenberger, Ribič-Pucelj, Sinkovec, Husen, Thole, Houba, Thaete, Adamski, Rižner: "Novel estrogen-related genes and potential biomarkers of ovarian endometriosis identified by differential expression analysis." in: The Journal of steroid biochemistry and molecular biology, Vol. 125, Issue 3-5, pp. 231-42, 2011 (Sample species: Human). Further details: Chromatin Immunoprecipitation
Mehasseb, Panchal, Taylor, Brown, Bell, Habiba: "Estrogen and progesterone receptor isoform distribution through the menstrual cycle in uteri with and without adenomyosis." in: Fertility and sterility, Vol. 95, Issue 7, pp. 2228-35, 2235.e1, 2011 (Sample species: Human). Further details: Chromatin Immunoprecipitation
Choijamts, Jimi, Kondo, Naganuma, Matsumoto, Kuroki, Iwasaki, Emoto: "CD133+ cancer stem cell-like cells derived from uterine carcinosarcoma (malignant mixed Müllerian tumor)." in: Stem cells (Dayton, Ohio), Vol. 29, Issue 10, pp. 1485-95, 2011 (Sample species: Human). Further details: Chromatin Immunoprecipitation
Chakravarty, Gupta, Goda, Srinivasan, Patel, Dhaliwal: "Steroid receptors, HER2/neu and Ki-67, in endometrioid type of endometrial carcinoma: Correlation with conventional histomorphological features of prognosis." in: Acta histochemica, Vol. 112, Issue 4, pp. 355-63, 2010 (Sample species: Human). Further details: Chromatin Immunoprecipitation
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