anti-Human Progesterone Receptor antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended Progesterone Receptor Antibody (supplied by: Log in to see )

Antigen
Progesterone Receptor (PGR) Antibodies
  • NR3C3
  • PR
  • 9930019P03Rik
  • BB114106
  • ENSMUSG00000074510
  • PR-A
  • PR-B
  • XPR-1
  • pgr
  • xpr-2
  • LOC443444
  • PGR
  • pg
  • pr
  • gb:dq017620
  • progesterone receptor
  • progesterone receptor L homeolog
  • PGR
  • Pgr
  • pgr.L
  • pgr
Epitope
AA 412-526
53
46
42
25
24
21
19
17
17
16
15
15
11
9
9
9
8
7
7
7
7
6
5
5
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
616
104
97
26
11
11
9
6
6
5
4
4
2
1
1
1
1
1
1
1
Host
Rabbit
331
295
3
1
Clonality
Monoclonal
Conjugate
This Progesterone Receptor antibody is un-conjugated
20
17
17
12
12
12
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
469
209
170
134
102
67
51
28
27
27
18
9
3
2
2
2
1
1
1
1
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Catalog No. ABIN1027517
$ 1,393.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1027518 IHC (f) IHC (p) Rabbit IgG AA 412-526 Log in to see SP42 0
1 ABIN6167071 IHC (f) CF®568 Mouse IgG1, kappa Log in to see PR500 0
1 ABIN6167019 IHC (f) CF®405S Mouse IgG1, kappa Log in to see PR484 0
1 ABIN6167023 IHC (f) CF®640R Mouse IgG1, kappa Log in to see PR484 0
1 ABIN6167025 IHC (f) CF®543 Mouse IgG1, kappa Log in to see PR484 0
1 ABIN6167033 IHC (f) CF®568 Mouse IgG1, kappa Log in to see PR484 0
1 ABIN6167043 IHC (f) CF®594 Mouse IgG1, kappa Log in to see PR484 0
1 ABIN6167039 IHC (f) CF®680R Mouse IgG1, kappa Log in to see PR484 0
1 ABIN6167057 IHC (f) CF®405S Mouse IgG1, kappa Log in to see PR500 0
1 ABIN6167065 IHC (f) CF®647 Mouse IgG1, kappa Log in to see PR500 0
1 ABIN6167073 IHC (f) CF®770 Mouse IgG1, kappa Log in to see PR500 0
1 ABIN6167079 IHC (f) CF®488A Mouse IgG1, kappa Log in to see PR500 0
1 ABIN6167081 IHC (f) CF®594 Mouse IgG1, kappa Log in to see PR500 0
1 ABIN6167095 IHC (f) WB CF®405S Mouse IgG1, kappa Log in to see PR501 0
1 ABIN6167097 IHC (f) WB CF405M Mouse IgG1, kappa Log in to see PR501 0
1 ABIN6167101 IHC (f) WB CF®543 Mouse IgG1, kappa Log in to see PR501 0
1 ABIN6167103 IHC (f) WB CF®647 Mouse IgG1, kappa Log in to see PR501 0
1 ABIN6167105 IHC (f) WB CF®555 Mouse IgG1, kappa Log in to see PR501 0
1 ABIN6167111 IHC (f) WB CF®770 Mouse IgG1, kappa Log in to see PR501 0
1 ABIN6167119 IHC (f) WB CF®594 Mouse IgG1, kappa Log in to see PR501 0

General

Antigen Progesterone Receptor (PGR) Antibodies
Epitope AA 412-526
(53), (46), (42), (25), (24), (21), (19), (17), (17), (16), (15), (15), (11), (9), (9), (9), (8), (7), (7), (7), (7), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(616), (104), (97), (26), (11), (11), (9), (6), (6), (5), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(331), (295), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Progesterone Receptor antibody is un-conjugated
(20), (17), (17), (12), (12), (12), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(469), (209), (170), (134), (102), (67), (51), (28), (27), (27), (18), (9), (3), (2), (2), (2), (1), (1), (1), (1)
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Product Details anti-Progesterone Receptor Antibody

Target Details Progesterone Receptor Application Details Handling Images
Purpose This antibody is designed for the specific localization of human PR using IHC techniques in formalin-fixed, paraffin- embedded tissue sections.
Characteristics The determination of steroid hormone receptors is of great importance for therapy of patients with hormone receptor expressing tumours. Progesterone and estrogen receptor positive breast carcinoma patients have demonstrated a better response to endocrine therapy than receptor negative patients. Expression of progesterone receptors in tumour cells of human breast carcinoma points to an intact estrogen receptor and to sensitivity of this tumour to endocrine therapy. Several investigations have shown that progesterone receptor is a better prognostic marker than estrogen receptor. Immunohistochemistry (IHC) is a complex technique in which immunological and histological detection methods are combined. In general, the manipulation and processing of tissues before immunostaining, especially different types of tissue fixation and embedding, as well as the nature of the tissues themselves may cause inconsistent results (Nadji and Morales, 1983). Endogenous pseudoperoxidase and peroxidase activity or endogenous biotin and alkaline phosphatase activity can cause non-specific staining results depending on the detection system used. Tissues that contain Hepatitis B surface antigen (HBsAg) can produce false positives when using HRP detection systems (Omata et al, 1980). Insufficient contrast staining and/or improper mounting of the sample may influence the interpretation of results.
Purification Ready-to-use, Prediluted, obtained from culture supernatant
Material not included All reagents, materials, and laboratory equipment for IHC procedures are not provided with this antibody. This includes adhesive slides and cover slips, positive and negative control tissues, Xylene or adequate substitute, ethanol, distilled H2O, heat pretreatment equipment (pressure cooker, steamer, microwave), pipettes, Coplin jars, glass jars, moist chamber, histological baths, negative control reagents, counter-staining solution, mounting materials, and microscope.
Immunogen Recombinant protein corresponding to amino acid residues 412-526 of human PR.
Clone SP42
Isotype IgG
Plasmids, Primers & others

Target Details Progesterone Receptor

Product Details anti-Progesterone Receptor Antibody Application Details Handling Images back to top
Antigen
Alternative Name Progesterone Receptor (PGR Antibody Abstract)
Pathways Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Smooth Muscle Cell Migration

Application Details

Product Details anti-Progesterone Receptor Antibody Target Details Progesterone Receptor Handling Images back to top
Comment

Staining pattern: Nuclear. The interpretation of the stain results is the full responsibility of the user. Any experimental result must be confirmed by a medically established diagnostic product or procedure.
Positive control: Tissue sample from breast carcinoma.
External Negative Control: Tissue sample homologous to the test sample incubated with an antibody isotype not specific for PR.

Protocol Principles of the procedure: The demonstrations of antigens by IHC is a sequential procedure with several steps involving first the application of a specific antibody for the antigen of interest (primary antibody), then a secondary antibody which joins to the first, an enzyme complex, and the addition of a chromogenic substrate. The sample is washed between each step. Enzymatic activation of the chromogenic substrate creates a visible product where the antigen is located. The results are interpreted using a light microscope. The primary antibody can be used both in manual IHC and with automated immunostainers.

Paraffin-embedded tissue samples should be used. The antibody is also useful for immunostaining frozen tissue samples. Western blot techniques are not recommended.
Assay Procedure Antigen retrieval: HIER Citrate Buffer pH 6.5
Working dilution: (only for concentrates) 1:50 – 1:200
Incubation: 30 min, RT
Control Tissue: Breast carcinoma
Restrictions For Research Use only

Handling

Product Details anti-Progesterone Receptor Antibody Target Details Progesterone Receptor Application Details Images back to top
Format Liquid
Buffer The preparation contains saline buffer, stabilising and carriers proteins. (pH 7.4)
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 2-8 °C until the expiration date printed on product label. Do not use after the expiration date. If fresh solutions are required, these must be prepared immediately prior to use, and will be stable for at least one day at room temperature (20-25°C). Unused portion of antibody preparation should be discarded after one day.If the product is stored under different conditions from those stipulated in these technical indications, the new conditions must be verified by the user. The validity period of the ready to use products when opened, is the same as the expiration date indicated on the label of intact product.

Images

Product Details anti-Progesterone Receptor Antibody Target Details Progesterone Receptor Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Progesterone Receptor (PGR) (AA 412-526) antibody (ABIN1027517) 10 × 20 × IHC of Progesterone Receptor (clone SP42) on FFPE breast carcinoma tissue. ...