anti-Rabbit PRMT2 antibody for Immunohistochemistry

Recommended PRMT2 Antibody (supplied by: Log in to see )

Antigen
Protein Arginine Methyltransferase 2 (PRMT2) Antibodies
  • PRMT2
  • DKFZp459N1919
  • HRMT1L1
  • Hrmt1l1
  • AI504737
  • protein arginine methyltransferase 2
  • protein arginine methyltransferase 2 L homeolog
  • protein arginine N-methyltransferase 2
  • PRMT2
  • Prmt2
  • prmt2.L
Epitope
N-Term
18
12
12
12
4
3
2
2
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
126
19
18
5
5
3
2
1
1
1
1
1
Host
Rabbit
83
29
15
Clonality
Polyclonal
Conjugate
This PRMT2 antibody is un-conjugated
5
5
5
5
5
5
Application
Immunohistochemistry (IHC), Western Blotting (WB)
118
57
42
31
11
4
3
2
2
1
1
Options
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Available images

Catalog No. ABIN2778663
$ 249.00
Plus shipping costs $45.00

General

Antigen Protein Arginine Methyltransferase 2 (PRMT2) Antibodies
Epitope N-Term
(18), (12), (12), (12), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(126), (19), (18), (5), (5), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(83), (29), (15)
Clonality
Polyclonal
Conjugate This PRMT2 antibody is un-conjugated
(5), (5), (5), (5), (5), (5)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(118), (57), (42), (31), (11), (4), (3), (2), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-PRMT2 Antibody

Target Details PRMT2 Application Details Handling References for anti-PRMT2 antibody (ABIN2778663) Images
Sequence ESQGEEPAEC SEAGLLQEGV QPEEFVAIAD YAATDETQLS FLRGEKILIL
Predicted Reactivity Cow: 100%, Dog: 93%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against PRMT2. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human PRMT2
Plasmids, Primers & others

Target Details PRMT2

Product Details anti-PRMT2 Antibody Application Details Handling References for anti-PRMT2 antibody (ABIN2778663) Images back to top
Antigen
Alternative Name PRMT2 (PRMT2 Antibody Abstract)
Background The protein arginine methyltransferases (PRMTs) include a family of proteins with related putative methyltransferase domains that modify chromatin and regulate cellular transcription. PRMT2 inhibits NF-kappaB-dependent transcription and promotes apoptosis and it exerts this effect by blocking nuclear export of IkappaB-alpha through a leptomycin-sensitive pathway, increasing nuclear IkappaB-alpha and decreasing NF-kappaB DNA binding. PRMT2 also rendered cells susceptible to apoptosis by cytokines or cytotoxic drugs, likely due to its effects on NF-kappaB.
Alias Symbols: HRMT1L1
Protein Interaction Partner: FIP1L1, SPAG8, RIPK2, BAG6, RB1, HNRNPA1, E2F1, UBC, PSMB3, HHV8GK18_gp81, CPSF7, DMRTB1, HNRNPUL1, PRMT2, THRB, PGR, NCOA6, NCOA1, ESR2, RXRA, PRRC2A, ESR1, PPARG, RARA,
Protein Size: 433
Molecular Weight 48 kDa
Gene ID 3275
NCBI Accession NM_001535, NP_001526
UniProt P55345
Pathways Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding

Application Details

Product Details anti-PRMT2 Antibody Target Details PRMT2 Handling References for anti-PRMT2 antibody (ABIN2778663) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 433 AA

Restrictions For Research Use only

Handling

Product Details anti-PRMT2 Antibody Target Details PRMT2 Application Details References for anti-PRMT2 antibody (ABIN2778663) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PRMT2 antibody (ABIN2778663)

Product Details anti-PRMT2 Antibody Target Details PRMT2 Application Details Handling Images back to top
Product cited in:

Burton, Muller, Tu, Padilla-Longoria, Guccione, Torres-Padilla: "Single-cell profiling of epigenetic modifiers identifies PRDM14 as an inducer of cell fate in the mammalian embryo." in: Cell reports, Vol. 5, Issue 3, pp. 687-701, 2013

Zhong, Cao, Zu, Hong, Yang, Liu, Xiao, Ding, Zhao, Liu, Wen: "Identification and characterization of novel spliced variants of PRMT2 in breast carcinoma." in: The FEBS journal, Vol. 279, Issue 2, pp. 316-35, 2012

Spellman, Ahmed, Dubach, Gardiner: "Expression of trisomic proteins in Down syndrome model systems." in: Gene, Vol. 512, Issue 2, pp. 219-25, 2012

Zhong, Cao, Hong, Yang, Zu, Xiao, Liu, Wen: "Identification and expression analysis of a novel transcript of the human PRMT2 gene resulted from alternative polyadenylation in breast cancer." in: Gene, Vol. 487, Issue 1, pp. 1-9, 2011

Background publications

Qi, Chang, Zhu, Yeldandi, Rao, Zhu: "Identification of protein arginine methyltransferase 2 as a coactivator for estrogen receptor alpha." in: The Journal of biological chemistry, Vol. 277, Issue 32, pp. 28624-30, 2002

Images

Product Details anti-PRMT2 Antibody Target Details PRMT2 Application Details Handling References for anti-PRMT2 antibody (ABIN2778663) back to top
Supplier Images
Western Blotting (WB) image for anti-Protein Arginine Methyltransferase 2 (PRMT2) (N-Term) antibody (ABIN2778663) WB Suggested Anti-PRMT2 Antibody Titration: 0.625ug/ml ELISA Titer: 1:1562500 Pos...
 image for anti-Protein Arginine Methyltransferase 2 (PRMT2) (N-Term) antibody (ABIN2778663) anti-Protein Arginine Methyltransferase 2 (PRMT2) (N-Term) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Protein Arginine Methyltransferase 2 (PRMT2) (N-Term) antibody (ABIN2778663) Rabbit Anti-PRMT2 Antibody Paraffin Embedded Tissue: Human Liver Cellular Data: Live...
Western Blotting (WB) image for anti-Protein Arginine Methyltransferase 2 (PRMT2) (N-Term) antibody (ABIN2778663) PRMT2 antibody - N-terminal region validated by WB using human LCL and mouse brains ...
Immunohistochemistry (IHC) image for anti-Protein Arginine Methyltransferase 2 (PRMT2) (N-Term) antibody (ABIN2778663) Rabbit Anti-PRMT2 Antibody Paraffin Embedded Tissue: Human cardiac cell Cell...