anti-Mouse (Murine) APAF1 antibody for Enzyme Immunoassay

Recommended APAF1 Antibody (supplied by: Log in to see )

Antigen
Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies
  • APAF1
  • ARK
  • Apaf-1
  • Apaf1
  • CG6829
  • D-Apaf-1
  • DARK
  • Dapaf-1
  • Dapaf-1/HAC-1
  • Dark
  • Dark/Apaf-I
  • Dark/Dapaf-1/HAC1
  • Dark/Hac-1/dApaf-1
  • Dark/Hac-1/dApaf1
  • Dmel\\CG6829
  • Hac-1
  • Hac-1/Dark
  • Hac1
  • T1
  • anon-53Fa
  • apaf-1
  • apaf1
  • arc
  • ark
  • dAPAF-1
  • dApaf-1
  • dApaf-1/DARK/HAC-1
  • dApaf1
  • dArk
  • dapaf
  • dapaf-1
  • dapaf-1L
  • dapaf-1S
  • dark
  • dark/dapaf-1/hac-1
  • dark/hac-1/dapaf-1
  • hac-1
  • hac1
  • l(2)SH0173
  • APAF-1
  • CED4
  • 6230400I06Rik
  • Apaf1l
  • fog
  • mKIAA0413
  • zgc:136543
  • Apaf-1-related-killer
  • apoptotic peptidase activating factor 1
  • apoptotic protease activating factor 1
  • Ark
  • apaf1
  • APAF1
  • Apaf1
Epitope
N-Term
45
41
17
13
13
8
5
5
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
201
129
110
7
4
3
3
3
2
1
Host
Rabbit
171
25
13
3
Clonality
Polyclonal
Conjugate
This APAF1 antibody is un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
164
72
63
59
36
23
22
17
7
4
3
3
2
Supplier
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Catalog No. ABIN5554538
$ 375.38
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5554539 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN5551027 EIA IHC (p) Mouse IgG C-Term Log in to see 5-00E-011

General

Antigen Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies
Epitope N-Term
(45), (41), (17), (13), (13), (8), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(201), (129), (110), (7), (4), (3), (3), (3), (2), (1)
Host Rabbit
(171), (25), (13), (3)
Clonality
Polyclonal
Conjugate This APAF1 antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(164), (72), (63), (59), (36), (23), (22), (17), (7), (4), (3), (3), (2)
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Product Details anti-APAF1 Antibody

Target Details APAF1 Application Details Handling Images
Specificity Apaf1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf1. The sequences of the immunogenic peptide are identical between human and mouse.
Purification Affinity chromatography purified via peptide column
Immunogen Human Apaf1 (N-Terminus) Peptide
Isotype IgG

Target Details APAF1

Product Details anti-APAF1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name APAF1 (APAF1 Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Apaf1 can also associate with caspase-4 and caspase-8 (4). Apaf1 is ubiquitously expressed in human tissues .Synonyms: Apaf-1, Apoptotic protease-activating factor 1, KIAA0413
Gene ID 317
UniProt O14727
Research Area Apoptosis/Necrosis, Cancer
Pathways p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-APAF1 Antibody Target Details APAF1 Handling Images back to top
Application Notes ELISA. Western Blot: Apaf1 antibody can be used for detection of Apaf1 at 1 - 2 μg/mL. Whole celllysate from HeLa cells can be used as positive control and a 115 - 130 kDa band should bedetected. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-APAF1 Antibody Target Details APAF1 Application Details Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.