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|Antigen||Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies|
|Epitope||AA 12-28 Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This APAF1 antibody is un-conjugated Alternatives|
Western Blotting (WB)
|6 references available|
|Supplier||Log in to see|
Product Details anti-APAF1 AntibodyTarget Details APAF1 Application Details Handling References for anti-APAF1 antibody (ABIN967648) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
|Purification||The polyclonal antibody was purified from antiserum by affinity chromatography.|
|Immunogen||Human APAF-1 aa. 12-28 Peptide|
Target Details APAF1Product Details anti-APAF1 Antibody Application Details Handling References for anti-APAF1 antibody (ABIN967648) Images back to top
|Alternative Name||Apaf-1 (APAF1 Antibody Abstract)|
Apoptosis can be induced by a family of cell death receptors (tumor necrosis factor) and their respective ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. Another mechanism of inducing apoptosis involves the participation of the mitochrondria in which various forms of cellular stress can trigger the release of cytochrome c from the intermembrane space (IMS) of the mitochrondria into the cytoplasm. The mammalian homologue of the CED-4 cell death gene in C. elegans has been identified from human and mouse and designated APAF-1 (for Apoptosis Protease Activating Factor-1). Cytochrome c associates with APAF-1 in the presence of dATP or ATP and induces an oligomerization of APAF-1 which then binds procaspase-9, forming an apoptosome” containing APAF-1, cytochrome c and procaspase-9. Recent studies have shown that dATP/ATP hydrolysis is necessary for APAF-1 to interact with cytochrome c and bind to procaspase-9. Procaspase-9 is then processed via an intrinsic autocatalytic activity of itself, triggered by APAF-1, which now allows caspase-9 to activate the downstream executioners caspase-3, -6 and -7. This antibody has been reported to recognize both human and mouse APAF-1. A peptide corresponding to amino acids 12 to 28 of human APAF-1 was used as the immunogen where the sequence of this peptide have been reported to be identical between both human and mouse.
Synonyms: Apoptosis Protease Activating Factor-1
|Molecular Weight||130 kDa|
|Pathways||p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-APAF1 Antibody Target Details APAF1 Handling References for anti-APAF1 antibody (ABIN967648) Images back to top
Related Products: ABIN968535, ABIN967390
|Restrictions||For Research Use only|
HandlingProduct Details anti-APAF1 Antibody Target Details APAF1 Application Details References for anti-APAF1 antibody (ABIN967648) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4° C.|
References for anti-APAF1 antibody (ABIN967648)Product Details anti-APAF1 Antibody Target Details APAF1 Application Details Handling Images back to top
|Product cited in:||
Hu, Benedict, Ding, Núñez: "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis." in: The EMBO journal, Vol. 18, Issue 13, pp. 3586-95, 1999
Cecconi, Alvarez-Bolado, Meyer, Roth, Gruss: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998
Green, Reed: "Mitochondria and apoptosis." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1309-12, 1998
Srinivasula, Ahmad, Fernandes-Alnemri, Alnemri: "Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization." in: Molecular cell, Vol. 1, Issue 7, pp. 949-57, 1998
Li, Nijhawan, Budihardjo, Srinivasula, Ahmad, Alnemri, Wang: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997
Zou, Henzel, Liu, Lutschg, Wang: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997
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