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|Antigen||BCL2 Binding Component 3 (BBC3) Antibodies|
|Epitope||AA 76-170 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This BBC3 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Product Details anti-BBC3 AntibodyTarget Details BBC3 Application Details Handling Images
|Specificity||Recognizes Human and Rat PUMA. Other species not tested.|
|Characteristics||Synonyms: BBC3, JFY-1, Bcl-2-binding component 3, p53 up-regulated modulator of apoptosis|
|Immunogen||A recombinant protein corresponding to amino acids 76-170 of Human PUMA-alpha|
Target Details BBC3Product Details anti-BBC3 Antibody Application Details Handling Images back to top
|Alternative Name||PUMA (BBC3 Antibody Abstract)|
|Background||Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis|
|Research Area||Apoptosis/Necrosis, Cancer, Metabolism|
|Pathways||p53 Signaling, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-BBC3 Antibody Target Details BBC3 Handling Images back to top
ELISA. Western Blot: PUMA antibody can be used for detection of PUMA at 2.5-5 μg/mL. Positive Control: K562 cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-BBC3 Antibody Target Details BBC3 Application Details Images back to top
|Buffer||PBS, 0.02 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.
|Expiry Date||12 months|