anti-Rat (Rattus) C-MYC antibody for Flow Cytometry

Recommended C-MYC Antibody (supplied by: Log in to see )

Antigen
V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antibodies
  • LOC100136746
  • RNCMYC
  • c-myc
  • mMyc
  • AU016757
  • Myc2
  • Niard
  • Nird
  • bHLHe39
  • MYC
  • cmyc
  • zc-myc
  • MRTL
  • MYCC
  • c-Myc
  • C-MYC
  • CMYC
  • CMyc
  • v-myc myelocytomatosis viral oncogene homolog
  • MYC proto-oncogene, bHLH transcription factor
  • myelocytomatosis oncogene
  • MYC proto-oncogene, bHLH transcription factor a
  • v-myc myelocytomatosis viral oncogene homolog (avian)
  • LOC100136746
  • Myc
  • myca
  • MYC
Reactivity
Human, Mouse (Murine), Rat (Rattus)
705
214
124
50
42
36
20
20
18
14
14
13
12
9
9
6
6
4
4
4
3
3
3
2
2
1
1
1
1
1
1
Host
Mouse
376
343
29
16
8
1
Clonality
Monoclonal
Conjugate
This C-MYC antibody is un-conjugated
47
41
40
21
17
17
14
13
13
13
13
13
13
12
12
12
10
10
8
5
5
5
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
483
244
229
226
226
160
150
124
92
52
18
14
14
11
10
8
6
6
4
2
2
1
Supplier
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Catalog No. ABIN3025109
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3197923 FACS IC IF IHC WB Rabbit N-Term Log in to see Polyclonal
1 ABIN4903082 FACS IF IHC IP WB Mouse IgG Log in to see
1 ABIN3025111 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see 9E10-3
1 ABIN2972869 FACS IF/ICC IHC WB Rabbit N-Term Log in to see Polyclonal

General

Antigen V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(705), (214), (124), (50), (42), (36), (20), (20), (18), (14), (14), (13), (12), (9), (9), (6), (6), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(376), (343), (29), (16), (8), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C-MYC antibody is un-conjugated
(47), (41), (40), (21), (17), (17), (14), (13), (13), (13), (13), (13), (13), (12), (12), (12), (10), (10), (8), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(483), (244), (229), (226), (226), (160), (150), (124), (92), (52), (18), (14), (14), (11), (10), (8), (6), (6), (4), (2), (2), (1)
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Product Details anti-C-MYC Antibody

Target Details C-MYC Application Details Handling Images
Characteristics It recognizes a transcription factor of 64-67 kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This mAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.
Purification Protein G affinity chromatography
Immunogen Amino acids AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA were used as the immunogen for the c-Myc antibody.
Clone 9E10-3
Isotype IgG1, kappa

Target Details C-MYC

Product Details anti-C-MYC Antibody Application Details Handling Images back to top
Antigen
Alternative Name C-Myc (MYC Antibody Abstract)
Background It recognizes a transcription factor of 64-67 kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This mAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.
Research Area Cancer, Cell Cycle, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus

Application Details

Product Details anti-C-MYC Antibody Target Details C-MYC Handling Images back to top
Application Notes Optimal dilution of the c-Myc antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL,Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the c-Myc antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) antibody (ABIN3025109) Formalin-fixed, paraffin-embedded human cervical carcinoma stained with c-Myc antibod...