anti-Chicken AKT2 antibody for Enzyme Immunoassay

Recommended AKT2 Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
  • AKT2
  • pkbb
  • pkbbeta
  • pkbss
  • prkbb
  • rac-beta
  • v-akt2
  • 2410016A19Rik
  • AW554154
  • PKB
  • PKBbeta
  • HIHGHH
  • PKBB
  • PKBBETA
  • PRKBB
  • RAC-BETA
  • cb945
  • akt2-a
  • AKT serine/threonine kinase 2
  • v-akt murine thymoma viral oncogene homolog 2
  • thymoma viral proto-oncogene 2
  • v-akt murine thymoma viral oncogene homolog 2 L homeolog
  • AKT2
  • akt2
  • Akt2
  • akt2.L
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis
456
166
127
37
33
27
26
25
22
22
21
21
21
4
3
3
2
1
1
Host
Rabbit
323
98
21
13
3
2
Clonality
Polyclonal
Conjugate
This AKT2 antibody is un-conjugated
21
21
18
18
17
15
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
392
251
172
97
63
56
33
26
19
13
10
8
7
4
3
3
2
2
1
1
Supplier
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Catalog No. ABIN5551333
$ 465.52
Plus shipping costs $45.00

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
Reactivity Chicken, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis
(456), (166), (127), (37), (33), (27), (26), (25), (22), (22), (21), (21), (21), (4), (3), (3), (2), (1), (1)
Host Rabbit
(323), (98), (21), (13), (3), (2)
Clonality
Polyclonal
Conjugate This AKT2 antibody is un-conjugated
(21), (21), (18), (18), (17), (15), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(392), (251), (172), (97), (63), (56), (33), (26), (19), (13), (10), (8), (7), (4), (3), (3), (2), (2), (1), (1)
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Product Details anti-AKT2 Antibody

Target Details AKT2 Application Details Handling Images
Specificity This antibody detects a ~65 kDa protein, corresponding to the Molecular Mass of Akt2 PKB beta on SDS-PAGE immunoblots. Does not react with PKB-alpha (Akt1) or PKB-gamma (Akt3).
Purification Affinity Chromatography
Immunogen A five residue synthetic peptide based on the Human Akt2, coupled to KLH
Isotype IgG

Target Details AKT2

Product Details anti-AKT2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name AKT2 / PKB beta (AKT2 Antibody Abstract)
Background Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).Synonyms: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase
Gene ID 208
NCBI Accession NP_001617
UniProt P31751
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling

Application Details

Product Details anti-AKT2 Antibody Target Details AKT2 Handling Images back to top
Application Notes Western blot (3): 1: 13000 dilution of SPC-148 was ufficient for detection of PKBβ in 20 μg ofHeLa cell lysate by ECL immunoblot analysis. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer TBS, 0.09 % Sodium Azide, 50 % Glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.