anti-Human AKT2 antibody for Cytometry by Time of Flight

Recommended AKT2 Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
  • AKT2
  • pkbb
  • pkbbeta
  • pkbss
  • prkbb
  • rac-beta
  • v-akt2
  • 2410016A19Rik
  • AW554154
  • PKB
  • PKBbeta
  • HIHGHH
  • PKBB
  • PKBBETA
  • PRKBB
  • RAC-BETA
  • cb945
  • akt2-a
  • AKT serine/threonine kinase 2
  • v-akt murine thymoma viral oncogene homolog 2
  • thymoma viral proto-oncogene 2
  • v-akt murine thymoma viral oncogene homolog 2 L homeolog
  • AKT2
  • akt2
  • Akt2
  • akt2.L
Epitope
AA 2-481
64
42
33
29
15
15
12
11
11
9
7
6
6
6
5
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
456
166
126
38
34
28
27
26
23
23
22
22
22
4
3
2
2
1
1
Host
Mouse
323
97
22
13
3
2
Clonality
Monoclonal
Conjugate
This AKT2 antibody is un-conjugated
21
21
18
18
17
15
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC)
384
241
163
92
62
51
32
27
18
13
11
8
7
4
3
3
2
2
1
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Catalog No. ABIN4899446
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General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
Epitope AA 2-481
(64), (42), (33), (29), (15), (15), (12), (11), (11), (9), (7), (6), (6), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(456), (166), (126), (38), (34), (28), (27), (26), (23), (23), (22), (22), (22), (4), (3), (2), (2), (1), (1)
Host Mouse
(323), (97), (22), (13), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT2 antibody is un-conjugated
(21), (21), (18), (18), (17), (15), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC)
(384), (241), (163), (92), (62), (51), (32), (27), (18), (13), (11), (8), (7), (4), (3), (3), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-AKT2 Antibody

Target Details AKT2 Application Details Handling References for anti-AKT2 antibody (ABIN4899446) Images
Specificity Detects human Akt2. Using direct ELISA, this antibody does not detect recombinant human (rh) Akt1 or rhAkt3.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Akt2, Asn2-Glu481, Accession # P31751
Clone 302501
Isotype IgG1

Target Details AKT2

Product Details anti-AKT2 Antibody Application Details Handling References for anti-AKT2 antibody (ABIN4899446) Images back to top
Antigen
Alternative Name Akt2 (AKT2 Antibody Abstract)
Background Alternative Names: EC 2.7.11, EC 2.7.11.1, Murine thymoma viral (v-akt) homolog-2, PKB beta, PKBB, PKBBETA, PRKBB, Protein kinase Akt-2, Protein kinase B beta, rac protein kinase beta, RAC-BETA, RAC-beta serine/threonine-protein kinase, RAC-PK-beta, v-akt murine thymoma viral oncogene homolog 2
Gene ID 208
UniProt P31751
Research Area Signaling, Cardiovascular, Atherosclerosis
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling

Application Details

Product Details anti-AKT2 Antibody Target Details AKT2 Handling References for anti-AKT2 antibody (ABIN4899446) Images back to top
Application Notes Immunohistochemistry 8-25 μg/mL
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details References for anti-AKT2 antibody (ABIN4899446) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-AKT2 antibody (ABIN4899446)

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling Images back to top
Product cited in:

Hagemann, Bozanovic, Hooper, Ljubic, Slettenaar, Wilson, Singh, Gayther, Shepherd, Van Trappen: "Molecular profiling of cervical cancer progression." in: British journal of cancer, Vol. 96, Issue 2, pp. 321-8, 2007 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Images

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling References for anti-AKT2 antibody (ABIN4899446) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 2-481) antibody (ABIN4899446) Detection of Akt2 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast canc...
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 2-481) antibody (ABIN4899446) Akt2 in Human Pancreatic Cancer Tissue. Akt2 was detected in immersion fixed paraffin...