anti-Pig (Porcine) AKT2 antibody for Immunocytochemistry

Recommended AKT2 Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
  • AKT2
  • pkbb
  • pkbbeta
  • pkbss
  • prkbb
  • rac-beta
  • v-akt2
  • 2410016A19Rik
  • AW554154
  • PKB
  • PKBbeta
  • HIHGHH
  • PKBB
  • PKBBETA
  • PRKBB
  • RAC-BETA
  • cb945
  • akt2-a
  • AKT serine/threonine kinase 2
  • v-akt murine thymoma viral oncogene homolog 2
  • thymoma viral proto-oncogene 2
  • v-akt murine thymoma viral oncogene homolog 2 L homeolog
  • AKT2
  • akt2
  • Akt2
  • akt2.L
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis
483
199
160
53
51
45
44
43
40
39
39
39
22
18
4
3
2
1
1
1
Host
Rabbit
356
96
18
14
3
2
Clonality
Polyclonal
Conjugate
This AKT2 antibody is conjugated to HRP
24
22
19
18
18
16
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA, Western Blotting (WB)
425
271
200
95
62
50
37
34
31
20
13
11
8
5
4
4
3
2
1
Options
Supplier
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Supplier Product No.
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Pubmed
3 references available

Available images

Immunofluorescence (fixed cells) (IF/ICC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody (HRP) (ABIN2486862)
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody (HRP) (ABIN2486862)
Catalog No. ABIN2486862
$ 452.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
18.461315 ABIN2486853 ICC IF ELISA WB Atto 594 Rabbit Log in to see Polyclonal 3
18.461315 ABIN2486858 ICC IF ELISA WB Alkaline Phosphatase (AP) Rabbit Log in to see Polyclonal 3
18.461315 ABIN2486866 ICC IF ELISA WB Streptavidin Rabbit Log in to see Polyclonal 3
18.461315 ABIN2486857 ICC IF ELISA WB Atto 700 Rabbit Log in to see Polyclonal 3
18.461315 ABIN2486865 ICC IF ELISA WB PE Rabbit Log in to see Polyclonal 3
18.461315 ABIN2486864 ICC IF ELISA WB PerCP Rabbit Log in to see Polyclonal 3
18.461315 ABIN2486860 ICC IF ELISA WB Biotin Rabbit Log in to see Polyclonal 3
18.461315 ABIN2486850 ICC IF ELISA WB Atto 390 Rabbit Log in to see Polyclonal 3
18.461315 ABIN2486855 ICC IF ELISA WB Atto 655 Rabbit Log in to see Polyclonal 3
18.461315 ABIN2486851 ICC IF ELISA WB Atto 488 Rabbit Log in to see Polyclonal 3
18.461315 ABIN2486854 ICC IF ELISA WB Atto 633 Rabbit Log in to see Polyclonal 3
18.461315 ABIN2486856 ICC IF ELISA WB Atto 680 Rabbit Log in to see Polyclonal 3
18.461315 ABIN2486863 ICC IF ELISA WB PE-Atto 594 Rabbit Log in to see Polyclonal 3
18.461315 ABIN2486859 ICC IF ELISA WB APC Rabbit Log in to see Polyclonal 3
18.461315 ABIN2486852 ICC IF ELISA WB Atto 565 Rabbit Log in to see Polyclonal 3
18.461315 ABIN2486861 ICC IF ELISA WB FITC Rabbit Log in to see Polyclonal 3
15.461315 ABIN361723 ICC IF ELISA WB Rabbit Log in to see Polyclonal 3

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
Reactivity Chicken, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis
(483), (199), (160), (53), (51), (45), (44), (43), (40), (39), (39), (39), (22), (18), (4), (3), (2), (1), (1), (1)
Host Rabbit
(356), (96), (18), (14), (3), (2)
Clonality
Polyclonal
Conjugate This AKT2 antibody is conjugated to HRP
(24), (22), (19), (18), (18), (16), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA, Western Blotting (WB)
(425), (271), (200), (95), (62), (50), (37), (34), (31), (20), (13), (11), (8), (5), (4), (4), (3), (2), (1)
Pubmed 3 references available
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Product Details anti-AKT2 Antibody

Target Details AKT2 Application Details Handling References for anti-AKT2 antibody (ABIN2486862) Images
Specificity Detects ~65 kDa. Does not react with PKBα (Akt1) or PKBγ (Akt3).
Purification Peptide Affinity Purified
Immunogen A five residue synthetic peptide from the C-terminal of human AKT2, coupled to KLH
Plasmids, Primers & others

Target Details AKT2

Product Details anti-AKT2 Antibody Application Details Handling References for anti-AKT2 antibody (ABIN2486862) Images back to top
Antigen
Alternative Name Akt2 (PKB Beta) (AKT2 Antibody Abstract)
Background Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).
Gene ID 208
NCBI Accession NP_001617
UniProt P31751
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling

Application Details

Product Details anti-AKT2 Antibody Target Details AKT2 Handling References for anti-AKT2 antibody (ABIN2486862) Images back to top
Application Notes
  • WB (1:1000)
  • ICC/IF (1:25)
  • optimal dilutions for assays should be determined by the user.
Comment

A 1:3000 dilution of SPC-148 was sufficient for detection of PKBβ in 20 μg of HeLa cell lysate by ECL immunoblot analysis.
Restrictions For Research Use only

Handling

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details References for anti-AKT2 antibody (ABIN2486862) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer TBS, 50 % glycerol, 0.01 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-AKT2 antibody (ABIN2486862)

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling Images back to top
Background publications

Nicholson, Anderson: "The protein kinase B/Akt signalling pathway in human malignancy." in: Cellular signalling, Vol. 14, Issue 5, pp. 381-95, 2002

Brazil, Hemmings: "Ten years of protein kinase B signalling: a hard Akt to follow." in: Trends in biochemical sciences, Vol. 26, Issue 11, pp. 657-64, 2001

Downward: "Mechanisms and consequences of activation of protein kinase B/Akt." in: Current opinion in cell biology, Vol. 10, Issue 2, pp. 262-7, 1998

Images

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling References for anti-AKT2 antibody (ABIN2486862) back to top
Supplier Images
Immunofluorescence (fixed cells) (IF/ICC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody (HRP) (ABIN2486862) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AKT2 Polyclonal Ant...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody (HRP) (ABIN2486862) Western blot analysis of multiple Cell line lysates showing detection of AKT2 protein...