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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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1 | ABIN2486855 | ICC IF ELISA WB | Atto 655 | Rabbit | Log in to see | Polyclonal | 3 | |||
1 | ABIN2486853 | ICC IF ELISA WB | Atto 594 | Rabbit | Log in to see | Polyclonal | 3 | |||
1 | ABIN2486854 | ICC IF ELISA WB | Atto 633 | Rabbit | Log in to see | Polyclonal | 3 | |||
1 | ABIN2486857 | ICC IF ELISA WB | Atto 700 | Rabbit | Log in to see | Polyclonal | 3 | |||
1 | ABIN2486858 | ICC IF ELISA WB | Alkaline Phosphatase (AP) | Rabbit | Log in to see | Polyclonal | 3 | |||
1 | ABIN2486860 | ICC IF ELISA WB | Biotin | Rabbit | Log in to see | Polyclonal | 3 | |||
1 | ABIN2486861 | ICC IF ELISA WB | FITC | Rabbit | Log in to see | Polyclonal | 3 | |||
1 | ABIN2486862 | ICC IF ELISA WB | HRP | Rabbit | Log in to see | Polyclonal | 3 | |||
1 | ABIN2486863 | ICC IF ELISA WB | PE-Atto 594 | Rabbit | Log in to see | Polyclonal | 3 | |||
1 | ABIN2486864 | ICC IF ELISA WB | PerCP | Rabbit | Log in to see | Polyclonal | 3 | |||
1 | ABIN2486865 | ICC IF ELISA WB | PE | Rabbit | Log in to see | Polyclonal | 3 | |||
1 | ABIN2486850 | ICC IF ELISA WB | Atto 390 | Rabbit | Log in to see | Polyclonal | 3 | |||
1 | ABIN2486856 | ICC IF ELISA WB | Atto 680 | Rabbit | Log in to see | Polyclonal | 3 | |||
1 | ABIN2486852 | ICC IF ELISA WB | Atto 565 | Rabbit | Log in to see | Polyclonal | 3 | |||
1 | ABIN2486859 | ICC IF ELISA WB | APC | Rabbit | Log in to see | Polyclonal | 3 | |||
1 | ABIN2486866 | ICC IF ELISA WB | Streptavidin | Rabbit | Log in to see | Polyclonal | 3 | |||
1 | ABIN361723 | ICC IF ELISA WB | Rabbit | Log in to see | Polyclonal | 3 |
General |
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Antigen | V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies |
Reactivity | Chicken, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis Alternatives |
Host | Rabbit Alternatives |
Clonality | |
Conjugate | This AKT2 antibody is conjugated to Atto 488 Alternatives |
Application |
Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA, Western Blotting (WB)
Alternatives
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3 references available |
Supplier | Log in to see |
Product Details anti-AKT2 AntibodyTarget Details AKT2 Application Details Handling References for anti-AKT2 antibody (ABIN2486851) Images |
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Specificity | Detects ~65 kDa. Does not react with PKBα (Akt1) or PKBγ (Akt3). |
Purification | Protein A Purified |
Immunogen | A five residue synthetic peptide based on the human Akt2, coupled to KLH |
Target Details AKT2Product Details anti-AKT2 Antibody Application Details Handling References for anti-AKT2 antibody (ABIN2486851) Images back to top |
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Antigen | |
Alternative Name | Akt2 (PKB Beta) (AKT2 Antibody Abstract) |
Background | Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3). |
Gene ID | 208 |
NCBI Accession | NP_001617 |
UniProt | P31751 |
Pathways | PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling |
Application DetailsProduct Details anti-AKT2 Antibody Target Details AKT2 Handling References for anti-AKT2 antibody (ABIN2486851) Images back to top |
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Application Notes |
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Comment |
A 1:3000 dilution of SPC-148 was sufficient for detection of PKBβ in 20 μg of HeLa cell lysate by ECL immunoblot analysis. |
Restrictions | For Research Use only |
HandlingProduct Details anti-AKT2 Antibody Target Details AKT2 Application Details References for anti-AKT2 antibody (ABIN2486851) Images back to top |
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Format | Liquid |
Concentration | 0.25 mg/mL |
Buffer | TBS, 50 % glycerol, 0.09 % sodium azide |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Storage | -20 °C |
References for anti-AKT2 antibody (ABIN2486851)Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling Images back to top |
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Background publications |
Nicholson, Anderson: "The protein kinase B/Akt signalling pathway in human malignancy." in: Cellular signalling, Vol. 14, Issue 5, pp. 381-95, 2002 Brazil, Hemmings: "Ten years of protein kinase B signalling: a hard Akt to follow." in: Trends in biochemical sciences, Vol. 26, Issue 11, pp. 657-64, 2001 Downward: "Mechanisms and consequences of activation of protein kinase B/Akt." in: Current opinion in cell biology, Vol. 10, Issue 2, pp. 262-7, 1998 |
ImagesProduct Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling References for anti-AKT2 antibody (ABIN2486851) back to top |
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Supplier Images |