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|Antigen||Cyclin-Dependent Kinase 2 (CDK2) Antibodies|
|Conjugate||This CDK2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|2 references available|
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Product Details anti-CDK2 AntibodyTarget Details CDK2 Application Details Handling References for anti-CDK2 antibody (ABIN359658) Images
|Specificity||This antibody reacts to CDK2.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CDK2.|
Target Details CDK2Product Details anti-CDK2 Antibody Application Details Handling References for anti-CDK2 antibody (ABIN359658) Images back to top
|Alternative Name||CDK2 (CDK2 Antibody Abstract)|
|Background||CDK2 is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation.Synonyms: Cell division protein kinase 2, Cyclin dependent kinase 2, p33 protein kinase|
|Gene ID||1017, 9606|
|Pathways||PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA|
Application DetailsProduct Details anti-CDK2 Antibody Target Details CDK2 Handling References for anti-CDK2 antibody (ABIN359658) Images back to top
ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CDK2 Antibody Target Details CDK2 Application Details References for anti-CDK2 antibody (ABIN359658) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-CDK2 antibody (ABIN359658)Product Details anti-CDK2 Antibody Target Details CDK2 Application Details Handling Images back to top
Moshinsky, Bellamacina, Boisvert, Huang, Hui, Jancarik, Kim, Rice: "SU9516: biochemical analysis of cdk inhibition and crystal structure in complex with cdk2." in: Biochemical and biophysical research communications, Vol. 310, Issue 3, pp. 1026-31, 2003
Chow, Siu, Ho, Ma, Ho, Poon: "Differential contribution of inhibitory phosphorylation of CDC2 and CDK2 for unperturbed cell cycle control and DNA integrity checkpoints." in: The Journal of biological chemistry, Vol. 278, Issue 42, pp. 40815-28, 2003
ImagesProduct Details anti-CDK2 Antibody Target Details CDK2 Application Details Handling References for anti-CDK2 antibody (ABIN359658) back to top