anti-Human PPP2R4 antibody for Flow Cytometry

Recommended PPP2R4 Antibody (supplied by: Log in to see )

Antigen
Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) Antibodies
  • 6330556D22Rik
  • PP2A
  • PR65
  • PR53
  • PTPA
  • PTPRA
  • 2610042B21Rik
  • C77440
  • N28142
  • protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform
  • protein phosphatase 2A activator, regulatory subunit 4
  • protein phosphatase 2A, regulatory subunit B (PR 53)
  • Ppp2r1a
  • PPP2R4
  • Ppp2r4
Reactivity
Human
80
36
31
6
5
3
3
2
2
2
2
2
1
1
Host
Mouse
40
33
11
Clonality
Monoclonal
Conjugate
This PPP2R4 antibody is un-conjugated
4
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
75
37
37
28
22
22
9
4
3
2
2
1
1
Options
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Catalog No. ABIN969557
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.060933 ABIN1108715 EIA FACS IF IHC (p) WB Mouse IgG1 Log in to see 4D9 2
14.060933 ABIN4347104 CyTOF ELISA FACS ICC IF IHC WB Mouse IgG1 Log in to see 4D9
14.060933 ABIN1844821 ELISA FACS IF/ICC IHC WB Mouse IgG1 Log in to see
1 ABIN1500384 ELISA FACS ICC IF IHC (p) WB Mouse IgG1 Log in to see 4D9
1 ABIN4880968 ELISA FACS ICC IHC WB Mouse IgG1 Log in to see 4D9
1 ABIN5632222 FACS Allophycocyanin Mouse IgG1 Log in to see 4D9
1 ABIN5632220 ELISA FACS ICC IF IHC WB Alexa Fluor 647 Mouse IgG1 Log in to see 4D9
1 ABIN5632218 ELISA FACS ICC IF IHC WB Alexa Fluor 405 Mouse IgG1 Log in to see 4D9
1 ABIN5632226 ELISA FACS ICC IF IHC WB DyLight 488 Mouse IgG1 Log in to see 4D9
1 ABIN5632225 ELISA FACS ICC IF IHC WB DyLight 405 Mouse IgG1 Log in to see 4D9
1 ABIN5632224 ELISA FACS ICC IF IHC WB DyLight 350 Mouse IgG1 Log in to see 4D9
1 ABIN5632227 ELISA FACS ICC IF IHC WB DyLight 550 Mouse IgG1 Log in to see 4D9
1 ABIN5632230 ELISA FACS ICC IF IHC WB DyLight 755 Mouse IgG1 Log in to see 4D9
1 ABIN5632231 ELISA FACS ICC IF IHC FITC Mouse IgG1 Log in to see 4D9
1 ABIN5632233 FACS PerCP Mouse IgG1 Log in to see 4D9
1 ABIN5632234 FACS PE Mouse IgG1 Log in to see 4D9
1 ABIN5632229 ELISA FACS ICC IF IHC WB DyLight 680 Mouse IgG1 Log in to see 4D9
1 ABIN5632228 ELISA FACS ICC IF IHC WB DyLight 650 Mouse IgG1 Log in to see 4D9
1 ABIN5632219 ELISA FACS ICC IF IHC WB Alexa Fluor 488 Mouse IgG1 Log in to see 4D9
1 ABIN5632221 ELISA FACS ICC IF IHC WB Alexa Fluor 700 Mouse IgG1 Log in to see 4D9

General

Antigen Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) Antibodies
Reactivity Human
(80), (36), (31), (6), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1)
Host Mouse
(40), (33), (11)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PPP2R4 antibody is un-conjugated
(4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(75), (37), (37), (28), (22), (22), (9), (4), (3), (2), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-PPP2R4 Antibody

Target Details PPP2R4 Application Details Handling References for anti-PPP2R4 antibody (ABIN969557) Images
Purification Purified
Immunogen Purified recombinant fragment of human PPP2R4 expressed in E. Coli.
Clone 4D9
Isotype IgG1

Target Details PPP2R4

Product Details anti-PPP2R4 Antibody Application Details Handling References for anti-PPP2R4 antibody (ABIN969557) Images back to top
Antigen
Alternative Name PPP2R4 (PPP2R4 Antibody Abstract)
Background Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms.
Molecular Weight 41 kDa
Gene ID 5524
HGNC 5524
Research Area Signaling
Pathways PI3K-Akt Signaling, M Phase, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-PPP2R4 Antibody Target Details PPP2R4 Handling References for anti-PPP2R4 antibody (ABIN969557) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only

Handling

Product Details anti-PPP2R4 Antibody Target Details PPP2R4 Application Details References for anti-PPP2R4 antibody (ABIN969557) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-PPP2R4 antibody (ABIN969557)

Product Details anti-PPP2R4 Antibody Target Details PPP2R4 Application Details Handling Images back to top
Product cited in:

Yoon, Jun, Park, Jun Yoo, Moon, Soon Baik, Kim, Kim, Kim, Koh, Taek Lee, You: "Optimal suppression of protein phosphatase 2A activity is critical for maintenance of human embryonic stem cell self-renewal." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 5, pp. 874-84, 2010

Singh, Ye, Bucur, Zhu, Tanya Santos, Rabinovitz, Wei, Gao, Hahn, Khosravi-Far: "Protein phosphatase 2A reactivates FOXO3a through a dynamic interplay with 14-3-3 and AKT." in: Molecular biology of the cell, Vol. 21, Issue 6, pp. 1140-52, 2010

Images

Product Details anti-PPP2R4 Antibody Target Details PPP2R4 Application Details Handling References for anti-PPP2R4 antibody (ABIN969557) back to top
Supplier Images
ELISA image for anti-Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) antibody (ABIN969557) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) antibody (ABIN969557) Figure 2: Flow cytometric analysis of MCF-7 cells using PPP2R4 mouse mAb (green) and ...
Immunocytochemistry (ICC) image for anti-Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) antibody (ABIN969557) Figure 3: Immunofluorescence analysis of Hela cells using PPP2R4 mouse mAb (green) . ...
Immunohistochemistry (IHC) image for anti-Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) antibody (ABIN969557) Figure 4: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues u...
Immunohistochemistry (IHC) image for anti-Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) antibody (ABIN969557) Figure 5: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using...
Western Blotting (WB) image for anti-Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) antibody (ABIN969557) Figure 6: Western blot analysis using PPP2R4 mAb against human PPP2R4 (AA: 1-154) rec...