anti-Mouse (Murine) MAP3K5 antibody for Western Blotting

Recommended MAP3K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
  • ASK1
  • MAPKKK5
  • MEKK5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
  • 7420452D20Rik
  • ASK
  • Mekk5
  • Ask1
  • RGD1306565
  • mitogen-activated protein kinase kinase kinase 5
  • MAP3K5
  • MAPKKK5
  • Map3k5
  • map3k5
Epitope
pSer966
68
52
30
22
19
17
15
15
15
11
10
8
7
6
6
5
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
409
181
102
5
1
1
Host
Rabbit
386
28
Clonality
Polyclonal
Conjugate
This MAP3K5 antibody is un-conjugated
12
11
10
5
5
5
5
5
5
5
5
5
5
5
5
4
4
2
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
317
168
137
81
65
50
25
17
12
11
8
4
2
1
1
Supplier
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Catalog No. ABIN6255571
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1357021 ABIN2705520 IHC WB Rabbit Center Log in to see Polyclonal 0
3.1357021 ABIN2707341 IHC WB Rabbit N-Term Log in to see Polyclonal 0
3.1357021 ABIN1531773 IHC ELISA WB Rabbit IgG AA 932-981, pSer966 Log in to see Polyclonal 2
3.1357021 ABIN1532802 IHC ELISA WB Rabbit IgG AA 932-981 Log in to see Polyclonal 2
3.1357021 ABIN1873617 WB Rabbit IgG Log in to see Polyclonal 0
3.1357021 ABIN1858085 ICC IHC WB Rabbit IgG AA 693-933 Log in to see Polyclonal 0
3.1357021 ABIN6271358 ELISA ICC IF WB Rabbit IgG pThr838 Log in to see Polyclonal 0
3.1357021 ABIN3181921 ELISA IHC WB Rabbit IgG pSer966 Log in to see Polyclonal 0
3.1357021 ABIN3183403 ELISA IHC WB Rabbit IgG Ser897 Log in to see Polyclonal 0
3.1357021 ABIN6260078 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
3.1357021 ABIN6260079 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
3.1357021 ABIN6260080 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 0
3.1357021 ABIN1870370 IHC WB Rabbit IgG pSer966 Log in to see Polyclonal 0
3.1357021 ABIN4281869 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal 0
3.1357021 ABIN1844367 IHC WB Rabbit pSer966 Log in to see Polyclonal 0
3.1357021 ABIN658363 IHC (p) WB Rabbit Ig Fraction AA 1175-1203, C-Term Log in to see Polyclonal 0
3.1357021 ABIN5958918 ELISA IHC (p) WB Rabbit IgG pSer966 Log in to see Polyclonal 0
3.1357021 ABIN2987571 IHC WB Rabbit IgG pSer966 Log in to see Polyclonal 0
3.1357021 ABIN1870368 WB Rabbit IgG pThr918 Log in to see Polyclonal 0
3.1357021 ABIN1870363 WB Rabbit IgG pSer1033 Log in to see Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
Epitope pSer966
(68), (52), (30), (22), (19), (17), (15), (15), (15), (11), (10), (8), (7), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(409), (181), (102), (5), (1), (1)
Host Rabbit
(386), (28)
Clonality
Polyclonal
Conjugate This MAP3K5 antibody is un-conjugated
(12), (11), (10), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (2), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(317), (168), (137), (81), (65), (50), (25), (17), (12), (11), (8), (4), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-MAP3K5 Antibody

Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 antibody (ABIN6255571) Images
Specificity Phospho-ASK1 (Ser966) Antibody detects endogenous levels of ASK1 only when phosphorylated at Serine 966
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human ASK1 around the phosphorylation site of Serine 966
Isotype IgG
Plasmids, Primers & others

Target Details MAP3K5

Product Details anti-MAP3K5 Antibody Application Details Handling References for anti-MAP3K5 antibody (ABIN6255571) Images back to top
Antigen
Alternative Name ASK1 (MAP3K5 Antibody Abstract)
Background

Description: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).

Gene: MAP3K5

Molecular Weight 155kDa
Gene ID 4217
UniProt Q99683
Pathways MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded protein response

Application Details

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling References for anti-MAP3K5 antibody (ABIN6255571) Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details References for anti-MAP3K5 antibody (ABIN6255571) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

References for anti-MAP3K5 antibody (ABIN6255571)

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling Images back to top
Product cited in:

Feng, Li, Du, Sun, Feng, Li, Liu, Wang, Liu, Amizuka, Li: "Dual function of peroxiredoxin I in lipopolysaccharide-induced osteoblast apoptosis via reactive oxygen species and the apoptosis signal-regulating kinase 1 signaling pathway." in: Cell death discovery, Vol. 4, pp. 47, 2018

Images

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 antibody (ABIN6255571) back to top
Supplier Images
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (pSer966) antibody (ABIN6255571) ABIN6267686 staining MDA-MB-435 by IF/ICC. The sample were fixed with PFA and permeab...
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (pSer966) antibody (ABIN6255571) Western blot analysis of ASK1 phosphorylation expression in TNF treated 293 whole cel...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (pSer966) antibody (ABIN6255571) ABIN6267686 at 1/100 staining human Lymph node tissue sections by IHC-P. The tissue w...
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (pSer966) antibody (ABIN6255571) Western blot analysis of Phospho-ASK1 (Ser966) expression in various lysates