Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 8-144) Protein Protein
- Protein Name
- Protein Type
- Protein Characteristics
- AA 8-144
- Tobacco (Nicotiana benthamiana)
- Cell Culture (CC), Western Blotting (WB)
- HHHHHHHHHH APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE
- Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific GM-CSF antibody.
- Molecular Formula: C699H1077N201O206S8
Isoelectric Point: 6,21
Extinction Coefficient: E 0.1 % (1g/L) = 0.898 (A 280 nm)
- Endotoxin Level
- < 0.04 EU/µg protein (LAL method)
- Protein Name
- Synonyms: Granulocyte-macrophage colony-stimulating factor (GM-CSF), Colony-stimulating factor (CSF), Molgramostin, Sargramostim
GMCSF is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. It´s involved in differentiation of dendritic cells and is a key factor in differentiation pathways leading form stem cells. GMCSF is produced by several cell types as monocytes, fibroblasts, endothelial cells and T- Lymphocytes in response to a number of inflammatory mediators present in the hemopoietic environment and peripheral site of inflammation. Human GMCF is an important therapeutic cytokine used in the treatment of myeloid leukemia, neutropenia and aplastic anemia and it could become interesting in the treatment following bone marrow transplantation. It performs its biological activity by binding to a specific receptor complex which is composed of a cytokine-specific alpha chain and beta chain, shared with the receptors for interleukin-3 and interleukin-5. GMCSR has been identified to mediate the activation of Jak-Stat and MAPK pathways.
- JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin
rHuman GM-CSF is a glycosylated polypeptide chain containing 127 amino acids (18-144 aa CSF2_HUMAN P04141 ), fused to 10 His tag at N-terminal. rHuman GM-CSF migrate as a broad band between 15 and 25 kDa due to post-translation modification, in particular glycosylation. Human recombinant protein expressed in Nicotiana benthamiana. Recombinant human Granulocyte-macrophage colony-stimulating factor (GM-CSF) contains a 10-His-tag at the N-terminal end, is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product contains no animal-derived components or impurities. Animal free product. The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with coomassie blue.
- For Research Use only
- Lyophilized protein should be reconstituted in water to a concentration of 25-50 ng /µL. Optimal concentration should be determined for specific application and cell lines.
- 10 mM PBS buffer pH 7.6 and 0.2 M NaCl
- 4 °C