TNF alpha Protein (AA 89-235)
-
- Target See all TNF alpha Proteins
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 89-235
-
Origin
- Mouse
-
Source
- Escherichia coli (E. coli)
- Purpose
- Recombinant Mouse Tumor Necrosis Factor/TNFα
- Sequence
- MDKPVAHVVA NHQVEEQLEW LSQRANALLA NGMDLKDNQL VVPADGLYLV YSQVLFKGQG CPDYVLLTHT VSRFAISYQE KVNLLSAVKS PCPKDTPEGA ELKPWYEPIY LGGVFQLEKG DQLSAEVNLP KYLDFAESGQ VYFGVIAL
- Characteristics
-
Recombinant Mouse Tumor Necrosis Factor/TNFα
The ED50 for this effect is typically 6.8 pg/mL.Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product TNF alpha Protein
-
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
-
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- Target
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- Alternative Name
- Tumor Necrosis Factor/Tnf (TNF alpha Products)
- Synonyms
- DIF Protein, TNF-alpha Protein, TNFA Protein, TNFSF2 Protein, RATTNF Protein, Tnfa Protein, tnf Protein, TNF-a Protein, TNFalpha Protein, Tnfsf1a Protein, TNFa Protein, cTNF Protein, Tnf-alpha Protein, tnfa-like Protein, TNF-ALPHA Protein, dif Protein, tnfa Protein, xtnf Protein, tnfsf2 Protein, tnf-alpha Protein, Cachectin Protein, tumor necrosis factor Protein, tumor necrosis factor b (TNF superfamily, member 2) Protein, tumor necrosis factor alpha Protein, tumor necrosis factor a (TNF superfamily, member 2) Protein, TNF Protein, Tnf Protein, tnf Protein, tnfb Protein, tnf-alpha Protein, LOC103694380 Protein, tnfa Protein
- Background
-
Recombinant Mouse Tumor Necrosis Factor/Tnf is produced with our E. coli expression system. The target protein is expressed with sequence (Asp89-Leu235) of Mouse Tnf.
Tumor Necrosis Factor (TNF) is a member of the Tumor Necrosis Factor family. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism. - Molecular Weight
- 16.4 kDa
- UniProt
- P06804
- Pathways
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
-