TNFSF9 Protein (AA 71-254) (His tag)

Catalog No. ABIN1880581

Product Details

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Protein Type: Recombinant

Protein Characteristics: AA 71-254

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This TNFSF9 protein is labelled with His tag.

Purpose: Recombinant Human 4-1BB Ligand/4-1BBL/TNFSF9/CD137L (C-6His)

Sequence: MREGPELSPD DPAGLLDLRQ GMFAQLVAQN VLLIDGPLSW YSDPGLAGVS LTGGLSYKED TKELVVAKAG VYYVFFQLEL RRVVAGEGSG SVSLALHLQP LRSAAGAAAL ALTVDLPPAS SEARNSAFGF QGRLLHLSAG QRLGVHLHTE ARARHAWQLT QGATVLGLFR VTPEIPAGLP SPRSELEHHH HHH

Characteristics: Recombinant Human Tumor necrosis factor ligand superfamily member 9/TNFSF9 is produced with our E. coli expression system. The target protein is expressed with sequence (Arg71-Glu254) of Human TNFSF9 fused with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Alternative Name: Tumor Necrosis Factor Ligand Superfamily Member 9/TNFSF9 (TNFSF9 Products)

Synonyms: TNFSF9 Protein, 4-1BB-L Protein, 4-1BBL Protein, AI848817 Protein, Cd137l Protein, Ly63l Protein, CD137L Protein, TNF superfamily member 9 Protein, tumor necrosis factor (ligand) superfamily, member 9 Protein, Tnfsf9 Protein, LOC476729 Protein, TNFSF9 Protein

Sub Type: Fusionprotein

Background: Tumor necrosis factor ligand superfamily member 9, also known as 4-1BBL, is a member of the the tumor necrosis factor family. It is a 254 amino acids cytokine that is expressed in brain, placenta, lung, skeletal muscle and kidney. TNFSF9 has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells, and is thought to be involved in T cell-tumor cell interaction.
Alternative Names: Tumor necrosis factor ligand superfamily member 9, 4-1BB ligand, 4-1BBL, TNFSF9.

Molecular Weight: 20.6 kDa

UniProt: P41273

Pathways: Activated T Cell Proliferation, Cancer Immune Checkpoints