CD25 Protein (Fc Tag)

Catalog No. ABIN7520179

This Recombinant CD25 protein is produced in HEK-293 Cells.

Quick Overview for CD25 Protein (Fc Tag) (ABIN7520179)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % by SDS-PAGE.

Product Details

Purification tag / Conjugate: This CD25 protein is labelled with Fc Tag.

Purpose: Recombinant Mouse IL-2-RA/CD25 Protein

Sequence: ELCLYDPPEV PNATFKALSY KNGTILNCEC KRGFRRLKEL VYMRCLGNSW SSNCQCTSNS HDKSRKQVTA QLEHQKEQQT TTDMQKPTQS MHQENLTGHC REPPPWKHED SKRIYHFVEG QSVHYECIPG YKALQRGPAI SICKMKCGKT GWTQPQLTCV DEREHHRFLA SEESQGSRNS SPESETSCPI TTTDFPQPTE TTAMTETFVL TMEYK

Specificity: Glu22-Lys236

Sterility: 0.22 μm filtered

Endotoxin Level: <0.1EU/μg

Biological Activity Comment: Measured by its binding ability in a functional ELISA.Immobilized APC anti-mouse CD25 Antibody at 1μg/mL (100 μL/well) can bind Mouse CD25 with a linear range of 0.46-6 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Target Details

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: IL-2-RA/CD25

Background:

Description: The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown.

Name: CD25, Il2r, Ly-43, p55, CD25, IL2R, IMD41, TCGFR, IDDM10

Gene ID: 16184

UniProt: P01590

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation