VEGF 165 (Active) Protein
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- Target
- VEGF 165
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Sequence
- APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
- Characteristics
- Biological activity was determined by the dose - dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0 - 8.0 ng/mL.
- Purity
- < 98 % by SDS-PAGE gel and HPLC analyses.
- Endotoxin Level
- Endotoxin level is less than 0.1 ng per μg (1 EU/μg).
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VEGF 165 protein
Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
VEGF 165 (AA 27-191) protein (His-Avi Tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
VEGF 165 (AA 27-191) (Active) protein (His tag,AVI tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
VEGF 165 (AA 27-191) protein (His-Avi Tag)Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
VEGF 165 (AA 207-371) protein (His tag)Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
VEGF 165 proteinOrigin: Rat Host: Escherichia coli (E. coli) Recombinant
VEGF 165 proteinOrigin: Mouse Host: Escherichia coli (E. coli) Recombinant
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Handling Advice
- As with any protein, exposing VEGF165 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
- Storage
- -20 °C
- Storage Comment
- The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted VEGF165 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.
- Expiry Date
- 24 months
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- Target
- VEGF 165
- Alternative Name
- VEGF165
- Background
- VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors , fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant human VEGF165 is a 38.2 kDa disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.
- Gene ID
- 7422
- NCBI Accession
- NP_001028928
- OMIM
- 76781487
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