CDK2 Protein (Transcript Variant 1) (His tag)
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- Target See all CDK2 Proteins
- CDK2 (Cyclin-Dependent Kinase 2 (CDK2))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CDK2 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human CDK2 (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- < 0.1 EU per μg protein as determined by LAL test
- Top Product
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Cyclin-Dependent Kinase 2 (CDK2) (AA 1-298) protein (GST tag)
CDK2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cyclin-Dependent Kinase 2 (CDK2) (AA 1-298) (Active) protein (GST tag)CDK2 Origin: Human Host: Insect cells (Sf9) Recombinant SDS, Func Active
Cyclin-Dependent Kinase 2 (CDK2) (AA 1-198) protein (His tag)CDK2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Cyclin-Dependent Kinase 2 (CDK2) (AA 211-298) protein (GST tag)CDK2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cyclin-Dependent Kinase 2 (CDK2) (AA 1-346) protein (Strep Tag)Crystallography grade CDK2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 20 mM Tris-HCl, 200 mM NaCl, 1 mM DTT, 40 % Glycerol, pH 8.0. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- CDK2 (Cyclin-Dependent Kinase 2 (CDK2))
- Alternative Name
- Cdk2 (CDK2 Products)
- Synonyms
- p33(CDK2) Protein, A630093N05Rik Protein, wu:fa10c02 Protein, zgc:56598 Protein, zgc:77684 Protein, eg1 Protein, CDKN2 Protein, cdk2L Protein, cyclin dependent kinase 2 Protein, cyclin-dependent kinase 2 Protein, cyclin-dependent kinase 2 S homeolog Protein, CDK2 Protein, Cdk2 Protein, cdk2 Protein, cdk2.S Protein
- Background
- This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.
- Molecular Weight
- 36.1 kDa
- NCBI Accession
- NP_001789
- Pathways
- PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
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