PRAME Protein (His tag)
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- Target See all PRAME Proteins
- PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))
- Protein Type
- Recombinant
- Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PRAME protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human PRAME / MAPE (full length, N-term HIS tag, transcript variant 5) protein expressed in E.coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product PRAME Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))
- Alternative Name
- Prame,mape (PRAME Products)
- Synonyms
- PRAME Protein, CT130 Protein, MAPE Protein, OIP-4 Protein, OIP4 Protein, 4930534P07Rik Protein, preferentially expressed antigen in melanoma Protein, PRAME Protein, Prame Protein
- Background
- This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.
- Molecular Weight
- 57.7 kDa
- NCBI Accession
- NP_996839
- Pathways
- Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding
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