BRAF Protein (AA 2-766) (His tag)

Catalog No. ABIN3090282

Product Details

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Protein Type: Recombinant

Protein Characteristics: AA 2-766

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This BRAF protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequence: AALSGGGGGG AEPGQALFNG DMEPEAGAGA GAAASSAADP AIPEEVWNIK QMIKLTQEHI EALLDKFGGE HNPPSIYLEA YEEYTSKLDA LQQREQQLLE SLGNGTDFSV SSSASMDTVT SSSSSSLSVL PSSLSVFQNP TDVARSNPKS PQKPIVRVFL PNKQRTVVPA RCGVTVRDSL KKALMMRGLI PECCAVYRIQ DGEKKPIGWD TDISWLTGEE LHVEVLENVP LTTHNFVRKT FFTLAFCDFC RKLLFQGFRC QTCGYKFHQR CSTEVPLMCV NYDQLDLLFV SKFFEHHPIP QEEASLAETA LTSGSSPSAP ASDSIGPQIL TSPSPSKSIP IPQPFRPADE DHRNQFGQRD RSSSAPNVHI NTIEPVNIDD LIRDQGFRGD GGSTTGLSAT PPASLPGSLT NVKALQKSPG PQRERKSSSS SEDRNRMKTL GRRDSSDDWE IPDGQITVGQ RIGSGSFGTV YKGKWHGDVA VKMLNVTAPT PQQLQAFKNE VGVLRKTRHV NILLFMGYST KPQLAIVTQW CEGSSLYHHL HIIETKFEMI KLIDIARQTA QGMDYLHAKS IIHRDLKSNN IFLHEDLTVK IGDFGLATVK SRWSGSHQFE QLSGSILWMA PEVIRMQDKN PYSFQSDVYA FGIVLYELMT GQLPYSNINN RDQIIFMVGR GYLSPDLSKV RSNCPKAMKR LMAECLKKKR DERPLFPQIL ASIELLARSL PKIHRSASEP SLNRAGFQTE DFSLYACASP KTPIQAGGYG AFPVH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human BRAF Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: BRAF (BRAF Products)

Synonyms: B-RAF1 Protein, BRAF1 Protein, NS7 Protein, RAFB1 Protein, C-RMIL Protein, RMIL Protein, B-Raf Protein, braf1 Protein, rafb1 Protein, b-raf1 Protein, sierrabraf Protein, BRAF Protein, 9930012E13Rik Protein, AA120551 Protein, AA387315 Protein, AA473386 Protein, B-raf Protein, Braf-2 Protein, Braf2 Protein, C230098H17 Protein, C87398 Protein, D6Ertd631e Protein, braf Protein, fc48g01 Protein, wu:fc48g01 Protein, B-Raf proto-oncogene, serine/threonine kinase Protein, B-Raf proto-oncogene, serine/threonine kinase S homeolog Protein, Braf transforming gene Protein, si:dkey-222f8.3 Protein, BRAF Protein, Braf Protein, braf.S Protein, braf Protein, si:dkey-222f8.3 Protein

Background: Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. {ECO:0000269|PubMed:21441910}.

Molecular Weight: 85.3 kDa Including tag.

UniProt: P15056

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy