BRAF Protein (Val600Glu-Mutant) (GST tag)
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- Target See all BRAF Proteins
- BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- Val600Glu-Mutant
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Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This BRAF protein is labelled with GST tag.
- Application
- SDS-PAGE (SDS), Functional Studies (Func)
- Purpose
- BRAF (V600E) (Human) Recombinant Protein
- Characteristics
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Human BRAF (NP_004324.2, 433 a.a. - 726 a.a.) V600E mutant partial recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
This product with activity data is belong to bioactive protein. - Purification
- Baculovirus expression system
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B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 433-726) (Active) protein (GST tag)
BRAF Origin: Human Host: Insect Cells Recombinant SDS, Func Active
B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 417-766) (Active) protein (His-GST)BRAF Origin: Human Host: Insect cells (Sf9) Recombinant SDS, Func Active
B-Raf proto-oncogene, serine/threonine kinase (BRAF) (Val600Glu-Mutant) (Active) protein (His-GST)BRAF Origin: Human Host: Insect cells (Sf9) Recombinant SDS, Func Active
B-Raf proto-oncogene, serine/threonine kinase (BRAF) (Active) protein (Myc-DYKDDDDK Tag)BRAF Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, STD, PI Active
B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 8-170) protein (His-IF2DI Tag)BRAF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 346-445) protein (GST tag)BRAF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 2-766) protein (His tag)Crystallography grade BRAF Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 2-804) protein (His tag)Crystallography grade BRAF Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
B-Raf proto-oncogene, serine/threonine kinase (BRAF) (Active) protein (DYKDDDDK Tag)BRAF Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, STD, PI Active
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Baculovirus expression system
Product Quality tested by: Loading 1 ug protein in SDS-PAGE - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05 % Brij35, 1 mM DTT, 10 % glycerol)
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
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- Target
- BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
- Alternative Name
- BRAF (BRAF Products)
- Synonyms
- B-RAF1 Protein, BRAF1 Protein, NS7 Protein, RAFB1 Protein, C-RMIL Protein, RMIL Protein, B-Raf Protein, braf1 Protein, rafb1 Protein, b-raf1 Protein, sierrabraf Protein, BRAF Protein, 9930012E13Rik Protein, AA120551 Protein, AA387315 Protein, AA473386 Protein, B-raf Protein, Braf-2 Protein, Braf2 Protein, C230098H17 Protein, C87398 Protein, D6Ertd631e Protein, braf Protein, fc48g01 Protein, wu:fc48g01 Protein, B-Raf proto-oncogene, serine/threonine kinase Protein, B-Raf proto-oncogene, serine/threonine kinase S homeolog Protein, Braf transforming gene Protein, si:dkey-222f8.3 Protein, BRAF Protein, Braf Protein, braf.S Protein, braf Protein, si:dkey-222f8.3 Protein
- Background
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Full Gene Name: v-raf murine sarcoma viral oncogene homolog B1
Synonyms: B-RAF1,BRAF1,FLJ95109,MGC126806,MGC138284,RAFB1 - Gene ID
- 673
- Pathways
- MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy
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