MECP2 Protein (AA 1-486) (His tag)

Catalog No. ABIN7312930

Product Details

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Protein Type: Recombinant

Protein Characteristics: AA 1-486

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This MECP2 protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Mass Spectrometry (MS)

Purpose: Recombinant Human MECP2 Protein is produced by our mammalian expression system and the target gene encoding Met1-Ser486 is expressed with a 6His tag at the C-terminus.

Sequence: MVAGMLGLRE EKSEDQDLQG LKDKPLKFKK VKKDKKEEKE GKHEPVQPSA HHSAEPAEAG KAETSEGSGS APAVPEASAS PKQRRSIIRD RGPMYDDPTL PEGWTRKLKQ RKSGRSAGKY DVYLINPQGK AFRSKVELIA YFEKVGDTSL DPNDFDFTVT GRGSPSRREQ KPPKKPKSPK APGTGRGRGR PKGSGTTRPK AATSEGVQVK RVLEKSPGKL LVKMPFQTSP GGKAEGGGAT TSTQVMVIKR PGRKRKAEAD PQAIPKKRGR KPGSVVAAAA AEAKKKAVKE SSIRSVQETV LPIKKRKTRE TVSIEVKEVV KPLLVSTLGE KSGKGLKTCK SPGRKSKESS PKGRSSSASS PPKKEHHHHH HHSESPKAPV PLLPPLPPPP PEPESSEDPT SPPEPQDLSS SVCKEEKMPR GGSLESDGCP KEPAKTQPAV ATAATAAEKY KHRGEGERKD IVSSSMPRPN REEPVDSRTP VTERVSVDHH HHHH

Purity: Greater than 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Target Details

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Alternative Name: MECP2 (MECP2 Products)

Synonyms: AUTSX3 Protein, MRX16 Protein, MRX79 Protein, MRXS13 Protein, MRXSL Protein, PPMX Protein, RS Protein, RTS Protein, RTT Protein, 1500041B07Rik Protein, BB130002 Protein, D630021H01Rik Protein, Mbd5 Protein, WBP10 Protein, wu:fk96a04 Protein, zgc:111857 Protein, methyl-CpG binding protein 2 Protein, methyl CpG binding protein 2 Protein, methyl-CpG binding protein 2 S homeolog Protein, MECP2 Protein, Mecp2 Protein, mecp2 Protein, mecp2.S Protein

Gene ID: 4204

UniProt: P51608

Pathways: Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane