GCK Protein (AA 1-321) (His tag)
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- Target See all GCK Proteins
- GCK (Glucokinase (Hexokinase 4) (GCK))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-321
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Origin
- E. coli
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GCK protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- 1-321 aa: MGSSHHHHHH SSGLVPRGSH MGSMTKYALV GDVGGTNARL ALCDIASGEI SQAKTYSGLD YPSLEAVIRV YLEEHKVEVK DGCIAIACPI TGDWVAMTNH TWAFSIAEMK KNLGFSHLEI INDFTAVSMA IPMLKKEHLI QFGGAEPVEG KPIAVYGAGT GLGVAHLVHV DKRWVSLPGE GGHVDFAPNS EEEAIILEIL RAEIGHVSAE RVLSGPGLVN LYRAIVKADN RLPENLKPKD ITERALADSC TDCRRALSLF CVIMGRFGGN LALNLGTFGG VFIAGGIVPR FLEFFKASGF RAAFEDKGRF KEYVHDIPVY LIVHDNPGLL GSGAHLRQTL GHIL
- Characteristics
- Purified Recombinant Glucokinase protein (His tagged)
- Purification
- Purified
- Purity
- > 95 % pure
- Biological Activity Comment
- Specific activity is > 70 units/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 umole of Glucose to D-glucose 6-phosphate per minute in the presence of Beta-NADP at pH 9.0 at 37C.
- Top Product
- Discover our top product GCK Protein
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Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (GST tag)
GCK Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Glucokinase (Hexokinase 4) (GCK) (AA 356-465) protein (GST tag)GCK Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (His tag)GCK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Glucokinase (Hexokinase 4) (GCK) proteinGCK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE.
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied in liquid form in 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 deg C. Avoid repeated freezing and thawing cycles.
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- Target
- GCK (Glucokinase (Hexokinase 4) (GCK))
- Alternative Name
- Glucokinase (GCK Products)
- Synonyms
- FGQTL3 Protein, GK Protein, GLK Protein, HHF3 Protein, HK4 Protein, HKIV Protein, HXKP Protein, LGLK Protein, MODY2 Protein, Gk Protein, Gls006 Protein, GCK Protein, BA4487 Protein, glk Protein, hk4 Protein, hhf3 Protein, hkiv Protein, hxkp Protein, gck-A Protein, mody2 Protein, glucokinase Protein, GLUKA Protein, RNGK2 Protein, glucokinase Protein, glucokinase (hexokinase 4) L homeolog Protein, glucokinase (hexokinase 4) Protein, GCK Protein, Gck Protein, glcK Protein, Bphyt_1025 Protein, Halhy_6548 Protein, gk Protein, gck.L Protein, gck Protein
- Pathways
- MAPK Signaling, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process
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